OmniVira

Structural and functional analysis of surface proteins from an A(H3N8) influenza virus isolated from New England harbor seals.

Hua Yang1 Ha T Nguyen2 Paul J Carney1 Zhu Guo1 Jessie C Chang1 Joyce Jones1 Charles T Davis1 Julie M Villanueva1 Larisa V Gubareva1 James Stevens3
Affiliations 3 institutions
  1. Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  2. Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA Battelle Memorial Institute, Atlanta, Georgia, USA.
  3. Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA [email protected].
PMID25540377 DOI10.1128/JVI.02723-14 Year2015 Journal Journal of virology J Virol Languageeng Statusppublish

Abstract

In late 2011, an A(H3N8) influenza virus infection resulted in the deaths of 162 New England harbor seals. Virus sequence analysis and virus receptor binding studies highlighted potential markers responsible for mammalian adaptation and a mixed receptor binding preference (S. J. Anthony, J. A. St Leger, K. Pugliares, H. S. Ip, J. M. Chan, Z. W. Carpenter, I. Navarrete-Macias, M. Sanchez-Leon, J. T. Saliki, J. Pedersen, W. Karesh, P. Daszak, R. Rabadan, T. Rowles, W. I. Lipkin, MBio 3:e00166-00112, 2012, http://dx.doi.org/10.1128/mBio.00166-12). Here, we present a detailed structural and biochemical analysis of the surface antigens of the virus. Results obtained with recombinant proteins for both the hemagglutinin and neuraminidase indicate a true avian receptor binding preference. Although the detection of this virus in new species highlights an increased potential for cross-species transmission, our results indicate that the A(H3N8) virus currently poses a low risk to humans. Cross-species transmission of zoonotic influenza viruses increases public health concerns. Here, we report a molecular and structural study of the major surface proteins from an A(H3N8) influenza virus isolated from New England harbor seals. The results improve our understanding of these viruses as they evolve and provide important information to aid ongoing risk assessment analyses as these zoonotic influenza viruses continue to circulate and adapt to new hosts.

Supporting text Virus Host Location
Virus Attachment 55 Amino Acid Sequence 128 Animals 1948 Antigens, Viral 49 Crystallography, X-Ray 32 Hemagglutinin Glycoproteins, Influenza Virus 180 Influenza A Virus, H3N8 Subtype 13 Microbial Sensitivity Tests 5 Models, Molecular 99 Molecular Sequence Data 160 Neuraminidase 62 New England 5 Orthomyxoviridae Infections 228 Phoca 2 Polysaccharides 31 Protein Binding 193 Protein Conformation 44 Recombinant Proteins 17 Sequence Alignment 51 Viral Proteins 152 NA protein, influenza A virus 12

Evidence records

1 total
Zoonotic Surveillance
1 records · 1 evidence types
Evidence type Virus Isolation
1 records
OVE1906 Virus Isolation
Key finding

An A(H3N8) influenza virus was isolated from New England harbor seals.

Virus
Influenza A virus H3N8
Host
harbor seal Phoca vitulina Natural host
Location
Not specified
Supporting text

Here, we report a molecular and structural study of the major surface proteins from an A(H3N8) influenza virus isolated from New England harbor seals.