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Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum).

Hirohito Ogawa1,2 Masahiro Kajihara3 Naganori Nao4 Asako Shigeno5 Daisuke Fujikura6 Bernard M Hang'ombe7 Aaron S Mweene8 Alisheke Mutemwa9 David Squarre10 Masao Yamada11 Hideaki Higashi12,13,14,15 Hirofumi Sawa16,17,18,19 Ayato Takada20,21,22
Affiliations 22 institutions
  1. Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. [email protected].
  2. Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  3. Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  4. Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  5. Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  6. Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  7. Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  8. Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  9. Provincial Veterinary Office, Department of Veterinary Services, Ministry of Fisheries and Livestock, P.O. Box 70416, Ndola 50100, Zambia. [email protected].
  10. Department of National Parks and Wildlife, Ministry of Tourism and Arts, Private Bag 1, Chilanga 10101, Zambia. [email protected].
  11. Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. [email protected].
  12. Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  13. Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  14. Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  15. Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  16. Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  17. Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  18. Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  19. Global Virus Network, 801 W Baltimore St, Baltimore, MD 21201, USA. [email protected].
  20. Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. [email protected].
  21. Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
  22. Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. [email protected].
PMID 29207524 2017 Viruses eng epublish
PubMed DOI Browse context

Article

Publication summary

Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (<i>Eidolon helvum</i>) in Zambia, a total of 107 spleen samples of <i>E. helvum</i> in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named <i>E. helvum</i> adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus <i>Mastadenovirus</i>. The virus shared similar characteristics of low G + C contents with recently isolated members of species <i>Bat mastadenoviruses E</i>, <i>F</i> and <i>G</i>, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name "<i>Bat mastadenovirus H</i>". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.

adenovirus bat Eidolon helvum Zambia Animals Base Composition Chiroptera Chlorocebus aethiops Cytopathogenic Effect, Viral DNA-Directed DNA Polymerase Genome, Viral Mastadenovirus Microscopy, Electron Open Reading Frames Phylogeny Sequence Analysis, DNA Sequence Homology Serotyping

Structured evidence records

Evidence records

2 total
Genomic Evolution 1 records
Genomic Evolution Extraction confidence 0.95
Key finding

Genome sequencing and phylogenetic analysis revealed that the bat adenovirus EhAdV 06-106 forms a new species within Mastadenovirus, proposed as Bat mastadenovirus H.

Location
Not specified
Supporting text

Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. ... According to the taxonomic criteria, we propose the tentative new species name 'Bat mastadenovirus H'.

Genes or proteins
DNA polymerase; open reading frames; whole genome
Analysis methods
whole genome sequencing; phylogenetic analysis; distance matrix analysis
Zoonotic Surveillance 1 records
Zoonotic Surveillance Extraction confidence 0.95
Key finding

Surveillance in straw-colored fruit bats in Zambia led to isolation of a novel bat adenovirus, EhAdV 06-106.

Virus
EhAdV
Location
Zambia Zambia
Supporting text

For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells.

Method
virus isolation; cell culture; next-generation sequencing
Sample type
spleen
Geographic raw
Zambia
Country inferred
Zambia