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Literature detail

Rousettus aegyptiacus Bats Do Not Support Productive Nipah Virus Replication.

Stephanie N Seifert1 Michael C Letko1 Trenton Bushmaker1 Eric D Laing2 Greg Saturday1 Kimberly Meade-White1 Neeltje van Doremalen1 Christopher C Broder2 Vincent J Munster1
Affiliations 2 institutions
  1. Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
  2. Uniformed Services University, Bethesda, Maryland, USA.
PMID 31682727 2020 J Infect Dis eng ppublish
PubMed DOI Browse context

Article

Publication summary

Nipah virus (NiV) is a bat-borne zoonotic pathogen that can cause severe respiratory distress and encephalitis upon spillover into humans. NiV is capable of infecting a broad range of hosts including humans, pigs, ferrets, dogs, cats, hamsters, and at least 2 genera of bats. Little is known about the biology of NiV in the bat reservoir. In this study, we evaluate the potential for the Egyptian fruit bat (EFB), Rousettus aegyptiacus, to serve as a model organism for studying NiV in bats. Our data suggest that NiV does not efficiently replicate in EFBs in vivo. Furthermore, we show no seroconversion against NiV glycoprotein and a lack of viral replication in primary and immortalized EFB-derived cell lines. Our data show that despite using a conserved target for viral entry, NiV replication is limited in some bat species. We conclude that EFBs are not an appropriate organism to model NiV infection or transmission in bats.

bats Egyptian fruit bats experimental infection Nipah virus Animals Chiroptera Henipavirus Infections Nipah Virus Species Specificity Virus Replication

Structured evidence records

Evidence records

4 total
Host Range Experiment 2 records
Host Range Experiment Extraction confidence 0.95
Key finding

Nipah virus failed to replicate efficiently in Rousettus aegyptiacus bats or in bat-derived cell lines, indicating limited host susceptibility.

Virus
NiV
Location
Not specified
Supporting text

Our data suggest that NiV does not efficiently replicate in EFBs in vivo. Furthermore, we show no seroconversion against NiV glycoprotein and a lack of viral replication in primary and immortalized EFB-derived cell lines.

Method
experimental infection; virus replication assay
Experimental system
in vivo animal experiment
Host Range Experiment Extraction confidence 0.95
Key finding

Nipah virus did not replicate in primary or immortalized Egyptian fruit bat-derived cell lines, indicating poor cellular permissiveness.

Virus
NiV
Location
Not specified
Supporting text

We show no seroconversion against NiV glycoprotein and a lack of viral replication in primary and immortalized EFB-derived cell lines.

Method
virus replication assay
Experimental system
in vitro cell culture
Receptor Usage 1 records
Receptor Usage Extraction confidence 0.70
Key finding

Nipah virus uses a conserved receptor for cell entry, but this receptor compatibility does not allow efficient replication in Rousettus aegyptiacus bats.

Virus
NiV
Location
Not specified
Supporting text

Our data show that despite using a conserved target for viral entry, NiV replication is limited in some bat species.

Receptors
conserved target for viral entry
Serological Evidence 1 records
Serological Evidence Extraction confidence 0.90
Key finding

Rousettus aegyptiacus bats did not seroconvert against Nipah virus glycoprotein, indicating absence of antibody response.

Virus
NiV
Location
Not specified
Supporting text

Furthermore, we show no seroconversion against NiV glycoprotein and a lack of viral replication in primary and immortalized EFB-derived cell lines.

Method
seroconversion assay
Sample type
serum