Straw-Colored Fruit Bats (<i>Eidolon helvum</i>) and Their Bat Flies (<i>Cyclopodia greefi</i>) in Nigeria Host Viruses with Multifarious Modes of Transmission.

<b><i>Background:</i></b> Bat flies (Diptera: Hippoboscoidea: <i>Nycteribiidae</i> and <i>Streblidae</i>) are increasingly appreciated as hosts of "bat-associated" viruses. We studied straw-colored fruit bats (<i>Eidolon helvum</i>) and their nycteribiid bat flies (<i>Cyclopodia greefi</i>) in Nigeria to investigate the role of bat flies in vectoring or maintaining viruses. <b><i>Methods:</i></b> We captured bats and bat flies across northern Nigeria. We used metagenomics to identify viruses in 40 paired samples (20 flies from 20 bats). We characterized viruses using genomic and phylogenetic methods, and we compared infection frequencies in bats and their bat flies. <b><i>Results:</i></b> In 20 bats, we detected two individuals (10%) infected with eidolon helvum parvovirus 1 (BtPAR4) (<i>Parvoviridae</i>; <i>Tetraparvovirus</i>), previously described in Ghana, and 10 bats (50%) with a novel parvovirus in the genus <i>Amdoparvovirus</i> (<i>Parvoviridae</i>). The amdoparvoviruses include Aleutian disease virus of mink and viruses of other carnivores but have not previously been identified in bats or in Africa. In 20 paired bat flies (each fly from 1 bat) all (100%) were infected with a novel virus in the genus <i>Sigmavirus</i> (<i>Rhabdoviridae</i>). The sigmaviruses include vertically transmitted viruses of dipterans. We did not detect BtPAR4 in any bat flies, and we did not detect the novel sigmavirus in any bats. However, we did detect the novel amdoparvovirus in 3 out of 20 bat flies sampled (15%), including in 2 bat flies from bats in which we did not detect this virus. <b><i>Discussion:</i></b> Our results show that bats and their bat flies harbor some viruses that are specific to mammals and insects, respectively, and other viruses that may transmit between bats and arthropods. Our results also greatly expand the geographic and host range of the amdoparvoviruses and suggest that some could be transmitted by arthropods. Bat flies may serve as biological vectors, mechanical vectors, or maintenance hosts for "bat-associated" viruses.