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Literature detail

Susceptibility of Livestock, Wildlife, and Domestic Host Species Cells to the 2022-2025 Reassortant Oropouche Virus.

Lindsey M Reister-Hendricks1 Dane C Jasperson2 Jessica Gutierrez1,3 Bethany L McGregor1 Stacey L P Scroggs1
Affiliations 3 institutions
  1. Arthropod-Borne Animal Diseases Research Unit, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS 66502, USA.
  2. Center for Grain and Animal Health Research, Agricultural Research Service, United States Department of Agriculture, Manhattan, KS 66502, USA.
  3. Department of Agricultural Resource Management, Wisconsin Department of Agriculture, Trade, and Consumer Protection, Madison, WI 53708, USA.
PMID 42075694 2026 Pathogens eng epublish
PubMed DOI Browse context

Article

Publication summary

Oropouche virus (OROV) is an emerging zoonotic arthropod-borne virus of public health importance. The host range of OROV is largely unknown, but antibody evidence suggests that wildlife and livestock species could be susceptible hosts. To identify potential North American mammalian reservoir hosts, OROV replication curves were generated using eight cell lines derived from livestock, wildlife, and domestic animal species (cow, sheep, bison, white-tailed deer, elk, pig, horse, and dog). The virus replicated in all cell lines by 48 h post infection, except for the horse cells. OROV replication success was greatest in the bison cells followed by pig and dog cells. Moderate replication was achieved in the deer, elk, sheep, and cow cells. These results indicate that numerous animal species may be susceptible hosts for OROV, including important agricultural and wildlife species, but pathogenesis studies are required to confirm this finding. Identifying the reservoir hosts for OROV will allow livestock producers, veterinarians, and public health officials to prepare appropriate vector and disease control measures should the virus initiate an outbreak in the United States.

animal arbovirus cell culture host susceptibility livestock OROV Orthobunyavirus virus replication curve wildlife Animals, Wild Bunyaviridae Infections Livestock Orthobunyavirus Reassortant Viruses Animals Animals, Domestic Cattle Cell Line

Structured evidence records

Evidence records

10 total
Host Range Experiment 8 records
Host Range Experiment Extraction confidence 0.98
Key finding

Experimental infection of horse-derived cells with Oropouche virus did not result in detectable replication within 48 hours, indicating lack of susceptibility in horse cells.

Virus
OROV
Location
Not specified
Supporting text

Oropouche virus (OROV) replication curves were generated using eight cell lines derived from livestock, wildlife, and domestic animal species (cow, sheep, bison, white-tailed deer, elk, pig, horse, and dog). The virus replicated in all cell lines by 48 h post infection, except for the horse cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Bison cells showed the highest replication levels of Oropouche virus among the tested species, demonstrating high cellular susceptibility.

Virus
OROV
Location
Not specified
Supporting text

OROV replication curves were generated using eight cell lines derived from livestock, wildlife, and domestic animal species (cow, sheep, bison, white-tailed deer, elk, pig, horse, and dog). The virus replicated in all cell lines by 48 h post infection, except for the horse cells. OROV replication success was greatest in the bison cells followed by pig and dog cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Pig cells supported high replication of Oropouche virus, suggesting strong susceptibility.

Virus
OROV
Host
pig cells
Location
Not specified
Supporting text

The virus replicated in all cell lines by 48 h post infection, except for the horse cells. OROV replication success was greatest in the bison cells followed by pig and dog cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Dog cells showed strong replication of Oropouche virus following infection, indicating susceptibility.

Virus
OROV
Host
dog cells
Location
Not specified
Supporting text

OROV replication success was greatest in the bison cells followed by pig and dog cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Moderate replication of Oropouche virus was observed in cow-derived cells, suggesting partial susceptibility.

Virus
OROV
Host
cow cells
Location
Not specified
Supporting text

Moderate replication was achieved in the deer, elk, sheep, and cow cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Moderate replication of Oropouche virus was detected in sheep-derived cells, demonstrating partial susceptibility.

Virus
OROV
Location
Not specified
Supporting text

Moderate replication was achieved in the deer, elk, sheep, and cow cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Oropouche virus achieved moderate replication in white-tailed deer cells, implying partial susceptibility.

Virus
OROV
Location
Not specified
Supporting text

Moderate replication was achieved in the deer, elk, sheep, and cow cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.98
Key finding

Elk-derived cells supported moderate replication of Oropouche virus, indicating partial susceptibility.

Virus
OROV
Host
elk cells
Location
Not specified
Supporting text

Moderate replication was achieved in the deer, elk, sheep, and cow cells.

Method
replication assay; cell infection
Experimental system
in vitro cell culture
Genomic Evolution 1 records
Genomic Evolution Extraction confidence 0.60
Key finding

The study investigates a reassortant Oropouche virus, implying genomic evolution through segment reassortment within Orthobunyavirus.

Virus
OROV
Host
Not specified
Location
Not specified
Supporting text

The title refers to the '2022-2025 Reassortant Oropouche Virus' and MeSH terms include 'Orthobunyavirus / genetics' and 'Reassortant Viruses / physiology', indicating genomic evidence of reassortment in Oropouche virus.

Recombination Or Reassortment 1 records
Recombination Or Reassortment Extraction confidence 0.90
Key finding

The study characterizes host cell susceptibility to a 2022–2025 reassortant Oropouche virus, confirming its reassortant nature.

Virus
OROV
Host
Not specified
Location
Not specified
Supporting text

The title refers to the '2022-2025 Reassortant Oropouche Virus', indicating that the virus examined is a reassortant strain.

Event type
reassortment