OmniVira

Literature detail

Development of a reverse genetics system enabling the rescue of recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1).

Wendy Howard1 Anna Hayman Angie Lackenby Alison Whiteley Brandon Londt Jill Banks John McCauley Wendy Barclay
Affiliations 1 institutions
  1. School of Biological Sciences, University of Reading, Berkshire RG6 6AJ, United Kingdom.
PMID 17494592 2007 Avian Dis eng ppublish
PubMed DOI Browse context

Article

Publication summary

We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.

Genetic Techniques Animals Cell Line Cricetinae DNA, Complementary DNA, Viral DNA-Directed RNA Polymerases Dogs Humans Influenza A Virus, H5N1 Subtype

Structured evidence records

Evidence records

2 total
Host Range Experiment 1 records
Host Range Experiment Extraction confidence 0.90
Key finding

A reverse genetics system was developed to rescue recombinant avian influenza virus A/Turkey/England/50-92/91 (H5N1) from cloned cDNA, supporting future studies of avian-human host range.

Virus
IAV H5N1
Location
Not specified
Supporting text

We have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.

Method
reverse genetics; virus rescue
Experimental system
reverse genetics
Molecular Adaptation 1 records
Molecular Adaptation Extraction confidence 0.90
Key finding

A reverse genetics system was developed for H5N1 avian influenza virus that permits targeted mutations in viral genes to study host range and pathogenesis.

Virus
IAV H5N1
Host
Not specified
Location
Not specified
Supporting text

This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.

Mechanism types
host_range; pathogenicity