OmniVira

Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus.

Ji-Young Min1 Celia Santos Adam Fitch Alan Twaddle Yoshiko Toyoda Jay V DePasse Elodie Ghedin Kanta Subbarao
Affiliations 1 institutions
  1. Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA.
PMID23864613 DOI10.1128/JVI.01016-13 Year2013 Journal Journal of virology J Virol Languageeng Statusppublish

Abstract

The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Supporting text Virus Host Location
Virulence 108 Virus Replication 191 Amino Acid Substitution 81 Animals 1948 Host Specificity 132 Influenza A Virus, H5N1 Subtype 300 Mice 253 Mutation 209 Orthomyxoviridae Infections 228 Respiratory System 21 Viral Proteins 152 PB2 protein, influenza virus 9

Evidence records

2 total
Functional Mechanism
2 records · 1 evidence types
Evidence type Molecular Adaptation
2 records
OVE1516 Molecular Adaptation
Key finding

The PB2 E627K substitution in avian H5N1 viruses confers virulence and broadens host range for infection of mammals.

Virus
Influenza A virus H5N1
Host
Not specified
Location
Not specified
Supporting text

The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals.

Genes or proteins
PB2 protein
Mutations
E627K
Mechanism types
virulence adaptation | host-range expansion
OVE1517 Molecular Adaptation
Key finding

An E-to-K host-adaptive mutation in the PB2 gene of avian H5N1 influenza virus emerged during infection of mice and correlated with efficient replication.

Virus
Influenza A virus H5N1
Host
Not specified
Location
Not specified
Supporting text

Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Genes or proteins
PB2 gene
Mutations
E-to-K
Mechanism types
replication adaptation