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Literature detail

Mammalian adaptation in the PB2 gene of avian H5N1 influenza virus.

Ji-Young Min1 Celia Santos Adam Fitch Alan Twaddle Yoshiko Toyoda Jay V DePasse Elodie Ghedin Kanta Subbarao
Affiliations 1 institutions
  1. Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland, USA.
PMID 23864613 2013 J Virol eng ppublish
PubMed DOI Browse context

Article

Publication summary

The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Virulence Virus Replication Amino Acid Substitution Animals Host Specificity Influenza A Virus, H5N1 Subtype Mice Mutation Orthomyxoviridae Infections Respiratory System Viral Proteins PB2 protein, influenza virus

Structured evidence records

Evidence records

2 total
Host Range Experiment 1 records
Host Range Experiment Extraction confidence 0.90
Key finding

Avian H5N1 influenza virus replicated efficiently in mice, acquiring the PB2 E627K mutation along the respiratory tract, demonstrating adaptation during mammalian infection.

Virus
IAV H5N1
Location
Not specified
Supporting text

The E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Method
experimental infection; virus replication assay
Sample type
respiratory tracts
Experimental system
in vivo animal experiment
Molecular Adaptation 1 records
Molecular Adaptation Extraction confidence 1.00
Key finding

The avian H5N1 influenza virus acquires the PB2 E627K mutation during replication in mice, promoting mammalian adaptation and efficient viral replication.

Virus
IAV H5N1
Host
Not specified
Location
Not specified
Supporting text

The substitution of glutamic acid (E) for lysine (K) at position 627 of the PB2 protein of avian H5N1 viruses has been identified as a virulence and host range determinant for infection of mammals. Here, we report that the E-to-K host-adaptive mutation in the PB2 gene appeared from day 4 and 5 along the respiratory tracts of mice and was complete by day 6 postinoculation. This mutation correlated with efficient replication of the virus in mice.

Genes or proteins
PB2
Mutations
E627K
Mechanism types
polymerase_activity; replication_efficiency; host_adaptation