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Literature detail

A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity.

Yohei Watanabe1 Tetsuo Ito2 Madiha S Ibrahim3 Yasuha Arai4 Kozue Hotta5,6 Hoang Vu Mai Phuong7 Nguyen Le Khanh Hang7 Le Quynh Mai7 Kosuke Soda8 Masaoki Yamaoka9 Emmanuel Djoko Poetranto10 Laksmi Wulandari10 Hiroaki Hiramatsu11 Tomo Daidoji12 Ritsuko Kubota-Koketsu13 Nongluk Sriwilaijaroen14,11 Takaaki Nakaya12 Yoshinobu Okuno13 Tadanobu Takahashi15 Takashi Suzuki15 Toshihiro Ito8 Hak Hotta9 Tetsu Yamashiro5,6 Tsukasa Hayashi2 Kouichi Morita16 Kazuyoshi Ikuta4 Yasuo Suzuki17
Affiliations 17 institutions
  1. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan. Electronic address: [email protected].
  2. KAINOS Laboratories, Inc., Tokyo 113-0033, Japan.
  3. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
  4. Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
  5. Center for Infectious Disease Research in Asia and Africa, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan
  6. Vietnam Research Station, Nagasaki University, c/o National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
  7. Department of Virology, National Institute of Hygiene and Epidemiology, Hanoi, Viet Nam.
  8. Avian Zoonosis Research Center, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
  9. Center for Infectious Diseases, Graduate School of Medicine, Kobe University, Hyogo 650-0017, Japan.
  10. Indonesia-Japan Collaborative Research Center, Institute of Tropical Disease, Airlangga University, Surabaya 60115, Indonesia.
  11. Health Science Hills, College of Life and Health Sciences, Chubu University, Aichi 487-8501, Japan.
  12. Department of Infectious Diseases, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
  13. Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kagawa 768-0061, Japan.
  14. Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand
  15. Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
  16. Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan.
  17. Health Science Hills, College of Life and Health Sciences, Chubu University, Aichi 487-8501, Japan. Electronic address: [email protected].
PMID 25461160 2015 Biosens Bioelectron eng ppublish
PubMed DOI Browse context

Article

Publication summary

A switch of viral hemagglutinin receptor binding specificity from bird-type α2,3- to human-type α2,6-linked sialic acid is necessary for an avian influenza virus to become a pandemic virus. In this study, an easy-to-use strip test to detect receptor binding specificity of influenza virus was developed. A biotinylated anti-hemagglutinin antibody that bound a broad range of group 1 influenza A viruses and latex-conjugated α2,3 (blue) and α2,6 (red) sialylglycopolymers were used in an immunochromatographic strip test, with avidin and lectin immobilized on a nitrocellulose membrane at test and control lines, respectively. Accumulation of a sialylglycopolymer-virus-antibody complex at the test line was visualized by eye. The strip test could be completed in 30min and did not require special equipment or skills, thereby avoiding some disadvantages of current methods for analyzing receptor binding specificity of influenza virus. The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans at viral titers >128 hemagglutination units. The strip test results were in agreement with those of ELISA virus binding assays, with correlations >0.95. In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.

Detection of receptor binding specificity Group 1 influenza A virus H5N1 avian influenza virus Immunochromatographic strip test Pandemic potential Sialylglycopolymer Animals Birds Chromatography, Affinity Equipment Design Hemagglutinin Glycoproteins, Influenza Virus Humans Influenza A virus Influenza A Virus, H5N1 Subtype Influenza in Birds Influenza, Human Reagent Strips

Structured evidence records

Evidence records

3 total
Molecular Adaptation 1 records
Molecular Adaptation Extraction confidence 0.90
Key finding

Variant H5N1 avian influenza viruses exhibited small increases in binding affinity to human-type α2,6 sialylglycans, indicating receptor binding adaptation toward humans.

Virus
IAV H5N1
Host
Not specified
Location
Not specified
Supporting text

The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans.

Genes or proteins
hemagglutinin
Receptors
α2,6 sialylglycans
Mechanism types
receptor_binding
Receptor Usage 1 records
Receptor Usage Extraction confidence 1.00
Key finding

Variant H5N1 avian influenza viruses showed increased binding affinity to human-type α2,6-linked sialylglycans detected using an immunochromatographic strip test.

Virus
IAV H5N1
Location
Not specified
Supporting text

The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans at viral titers >128 hemagglutination units.

Method
immunochromatographic strip test; ELISA virus binding assay
Receptors
α2,6 sialylglycans
Zoonotic Surveillance 1 records
Zoonotic Surveillance Extraction confidence 0.70
Key finding

An immunochromatographic strip test for group 1 avian influenza A viruses enables field monitoring of receptor specificity changes associated with zoonotic risk.

Virus
IAV
Host
Aves
Location
Not specified
Supporting text

In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.

Method
immunochromatographic strip test