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Literature detail

Sensitive and direct detection of receptor binding specificity of highly pathogenic avian influenza A virus in clinical samples.

Tadanobu Takahashi1 Tatsuya Kawakami Takashi Mizuno Akira Minami Yuko Uchida Takehiko Saito Shigeyuki Matsui Makoto Ogata Taichi Usui Nongluk Sriwilaijaroen Hiroaki Hiramatsu Yasuo Suzuki Takashi Suzuki
Affiliations 1 institutions
  1. Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Shizuoka, Japan.
PMID 24205123 2013 PLoS One eng epublish
PubMed DOI Browse context

Article

Publication summary

Influenza A virus (IAV) recognizes two types of N-acetylneuraminic acid (Neu5Ac) by galactose (Gal) linkages, Neu5Acα2,3Gal and Neu5Acα2,6Gal. Avian IAV preferentially binds to Neu5Acα2,3Gal linkage, while human IAV preferentially binds to Neu5Acα2,6Gal linkage, as a virus receptor. Shift in receptor binding specificity of avian IAV from Neu5Acα2,3Gal linkage to Neu5Acα2,6Gal linkage is generally believed to be a critical factor for its transmission ability among humans. Surveillance of this shift of highly pathogenic H5N1 avian IAV (HPAI) is thought to be a very important for prediction and prevention of a catastrophic pandemic of HPAI among humans. In this study, we demonstrated that receptor binding specificity of IAV bound to sialo-glycoconjugates was sensitively detected by quantifying the HA gene with real-time reverse-transcription-PCR. The new assay enabled direct detection of receptor binding specificity of HPAIs in chicken clinical samples including trachea and cloaca swabs in only less than 4 h.

Animals Birds Glycoconjugates Hemagglutinin Glycoproteins, Influenza Virus Humans Influenza A virus Influenza A Virus, H5N1 Subtype Influenza in Birds N-Acetylneuraminic Acid Protein Binding Receptors, Virus

Structured evidence records

Evidence records

2 total
Receptor Usage 1 records
Receptor Usage Extraction confidence 1.00
Key finding

Influenza A viruses exhibit receptor binding specificity, with avian strains binding Neu5Acα2,3Gal and human strains binding Neu5Acα2,6Gal; a shift toward human-type receptor usage in avian H5N1 is associated with increased transmissibility among humans.

Virus
IAV H5N1
Location
Not specified
Supporting text

Avian IAV preferentially binds to Neu5Acα2,3Gal linkage, while human IAV preferentially binds to Neu5Acα2,6Gal linkage, as a virus receptor. Shift in receptor binding specificity of avian IAV from Neu5Acα2,3Gal linkage to Neu5Acα2,6Gal linkage is generally believed to be a critical factor for its transmission ability among humans.

Method
real-time reverse-transcription-PCR; receptor binding assay based on sialo-glycoconjugates
Receptors
Neu5Acα2,3Gal / Neu5Acα2,6Gal
Zoonotic Surveillance 1 records
Zoonotic Surveillance Extraction confidence 0.90
Key finding

An RT-PCR assay detected receptor binding specificity of highly pathogenic avian influenza A virus in chicken trachea and cloaca swabs.

Virus
IAV
Location
Not specified
Supporting text

The new assay enabled direct detection of receptor binding specificity of HPAIs in chicken clinical samples including trachea and cloaca swabs in only less than 4 h.

Method
real-time reverse-transcription-PCR
Sample type
trachea swab; cloaca swab