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Literature detail

Sparse serological evidence of H5N1 avian influenza virus infections in domestic cats, northeastern China.

Lingshuang Sun1,2 Pei Zhou1,3 Shuyi He4 Yongfeng Luo1,3 Kun Jia1,3 Cheng Fu1,3 Yao Sun1,3 Huamei He1,3 Liqing Tu1,3 Zhangyong Ning1,3 Ziguo Yuan1,3 Heng Wang1,3 Shoujun Li1,3 Liguo Yuan1,5
Affiliations 5 institutions
  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China
  2. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China. Electronic address: [email protected].
  3. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China.
  4. College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province 510642, People's Republic of China.
  5. Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, Guangdong Province 510642, People's Republic of China. Electronic address: [email protected].
PMID 25752867 2015 Microb Pathog eng ppublish
PubMed DOI Browse context

Article

Publication summary

Today the cross-species transmission of avian influenza viruses (AIV) are a great concern. A number of AIV strains are now enzootic among poultry, with H9N2 and highly pathogenic H5N1 AIV strains prevalent in China. H5N1 strains have been recognized to infect zoo and domestic feline species. In this serological study we sought to examine evidence that H5N1 strains have infected domestic cats in northeastern China. In 2013, we conducted a cross-sectional serological study of 916 healthy cats in Heilongjian, Jilin, and Liaonin Provinces. Sera were screened with a hemagglutinin inhibition (HI) assay and seropositive specimens (HI ≥ 1:20) were further evaluated with a microneutralization (MN) assay against a clade 2.3.2 H5N1 AIV, a H9N2 AIV, A (H1N1)pdm09, and a canine H3N2 virus. While ∼2% of cats had elevated HI assays against H5N1, no elevations were confirmed (MN ≥ 1:80). These data serve as baseline for future surveillance for AIV infections among domestic cats. Conducting such surveillance seems important for geographical areas recognized as endemic for AIVs. This is especially true for countries such as China where domestic cats and poultry are often in close contact.

Cats H5N1 Infection Animals Antibodies, Neutralizing Antibodies, Viral Cat Diseases Cats China Cross-Sectional Studies Hemagglutination Inhibition Tests Influenza A Virus, H3N2 Subtype Influenza A Virus, H5N1 Subtype Influenza A Virus, H9N2 Subtype Neutralization Tests Orthomyxoviridae Infections Seroepidemiologic Studies

Structured evidence records

Evidence records

3 total
Cross Species Transmission 1 records
Cross Species Transmission Extraction confidence 0.80
Key finding

Serological testing in northeastern China suggested possible transmission of H5N1 avian influenza virus from avian sources to domestic cats.

Virus
IAV H5N1
Location
China northeastern China, China
Supporting text

H5N1 strains have been recognized to infect zoo and domestic feline species. In this serological study we sought to examine evidence that H5N1 strains have infected domestic cats in northeastern China.

Method
hemagglutination inhibition assay; microneutralization assay
Study design
serological survey
Transmission direction
animal-to-animal
Geographic raw
northeastern China
Country inferred
China
Serological Evidence 1 records
Serological Evidence Extraction confidence 1.00
Key finding

About 2% of domestic cats in northeastern China showed elevated HI titers against H5N1 avian influenza virus, but none were confirmed by microneutralization assay, indicating sparse serological evidence of infection.

Virus
IAV H5N1
Location
Not specified
Supporting text

In 2013, we conducted a cross-sectional serological study of 916 healthy cats in Heilongjian, Jilin, and Liaonin Provinces. Sera were screened with a hemagglutinin inhibition (HI) assay and seropositive specimens (HI ≥ 1:20) were further evaluated with a microneutralization (MN) assay against a clade 2.3.2 H5N1 AIV. While ∼2% of cats had elevated HI assays against H5N1, no elevations were confirmed (MN ≥ 1:80).

Method
hemagglutination inhibition assay; microneutralization assay
Sample type
sera
Zoonotic Surveillance 1 records
Zoonotic Surveillance Extraction confidence 0.90
Key finding

Domestic cats in northeastern China were surveyed serologically for H5N1 avian influenza virus exposure, with few positive results providing baseline data for zoonotic surveillance.

Virus
IAV H5N1
Location
China China
Supporting text

In 2013, we conducted a cross-sectional serological study of 916 healthy cats in Heilongjian, Jilin, and Liaonin Provinces. Sera were screened with a hemagglutinin inhibition (HI) assay and seropositive specimens were further evaluated with a microneutralization (MN) assay against a clade 2.3.2 H5N1 AIV.

Method
hemagglutinin inhibition (HI) assay; microneutralization (MN) assay; serological study
Sample type
sera
Geographic raw
Heilongjian, Jilin, and Liaonin Provinces, northeastern China
Country inferred
China