OmniVira

Literature detail

Serological evidence of human infection by bat orthoreovirus in Singapore.

Anna Uehara1 Chee Wah Tan1 Shailendra Mani1 Kaw Bing Chua2 Yee Sin Leo3 Danielle E Anderson1 Lin-Fa Wang1
Affiliations 3 institutions
  1. Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore.
  2. Molecular Pathogenesis Group, Temasek Lifesciences Laboratory, National University of Singapore, Singapore.
  3. Institute of Infectious Disease and Epidemiology, Communicable Disease Centre, Tan Tock Seng Hospital, Singapore.
PMID 30411364 2019 J Med Virol eng ppublish
PubMed DOI Browse context

Article

Publication summary

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays. Our findings confirm that PRV spillover into human populations is relatively common in this region of the world.

bat luciferase immunoprecipitation system (LIPS) orthoreovirus spillover virus neutralization test (VNT) Adult Aged Aged, 80 and over Animals Antibodies, Viral Female Humans Longitudinal Studies Male Middle Aged Orthoreovirus Reoviridae Infections Seroepidemiologic Studies

Structured evidence records

Evidence records

2 total
Serological Evidence 1 records
Serological Evidence Extraction confidence 1.00
Key finding

At least seven individuals in Singapore had specific antibodies against Pteropine orthoreovirus detected by LIPS and confirmed by neutralization test, demonstrating human infection and spillover from bats.

Location
Not specified
Supporting text

We tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays.

Method
luciferase immunoprecipitation system; virus neutralization test
Sample type
serum
Spillover Event 1 records
Spillover Event Extraction confidence 0.95
Key finding

Human serological evidence indicates that Pteropine orthoreovirus has spilled over from bats to humans in Singapore.

Location
Singapore Singapore
Supporting text

Our findings confirm that PRV spillover into human populations is relatively common in this region of the world.

Method
luciferase immunoprecipitation system; virus neutralization test
Study design
serological survey
Transmission direction
animal-to-human
Geographic raw
Singapore
Country inferred
Singapore