OmniVira

Serological evidence of human infection by bat orthoreovirus in Singapore.

Anna Uehara1 Chee Wah Tan1 Shailendra Mani1 Kaw Bing Chua2 Yee Sin Leo3 Danielle E Anderson1 Lin-Fa Wang1
Affiliations 3 institutions
  1. Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore.
  2. Molecular Pathogenesis Group, Temasek Lifesciences Laboratory, National University of Singapore, Singapore.
  3. Institute of Infectious Disease and Epidemiology, Communicable Disease Centre, Tan Tock Seng Hospital, Singapore.
PMID30411364 DOI10.1002/jmv.25355 Year2019 Journal Journal of medical virology J Med Virol Languageeng Statusppublish

Abstract

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays. Our findings confirm that PRV spillover into human populations is relatively common in this region of the world.

Supporting text Virus Host Location
bat 54 luciferase immunoprecipitation system (LIPS) 1 orthoreovirus 6 spillover 105 virus neutralization test (VNT) 1 Adult 71 Aged 31 Aged, 80 and over 8 Animals 1949 Antibodies, Viral 212 Female 289 Humans 1441 Longitudinal Studies 17 Male 224 Middle Aged 60 Orthoreovirus 6 Reoviridae Infections 7 Seroepidemiologic Studies 113 Serologic Tests 8 Singapore 5 Young Adult 26 Zoonoses 397

Evidence records

2 total
Zoonotic Surveillance
2 records · 1 evidence types
Evidence type Serological Evidence
2 records
OVE3134 Serological Evidence
Key finding

At least seven humans in Singapore had specific antibodies against Pteropine orthoreovirus, indicating prior exposure.

Virus
Orthoreovirus
Host
human Homo sapiens Natural host
Location
Not specified
Supporting text

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013. After an initial screen with luciferase immunoprecipitation system and secondary confirmation with virus neutralization test, we identified at least seven individuals with specific antibodies against PRV in both assays.

Method
luciferase immunoprecipitation system | virus neutralization test
Sample type
serum samples
OVE3135 Serological Evidence
Key finding

Serological detection of antibodies against Pteropine orthoreovirus in humans indicates bat-to-human spillover in Singapore.

Virus
Orthoreovirus
Host
bats Chiroptera Natural host human Homo sapiens Natural host
Location
Singapore
Supporting text

To determine whether Pteropine orthoreovirus (PRV) exposure has occurred in Singapore, we tested 856 individuals from an existing serum panel collected from 2005-2013.

Method
luciferase immunoprecipitation system | virus neutralization test