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Literature detail

A serological survey of SARS-CoV-2 in cat in Wuhan.

Qiang Zhang1 Huajun Zhang2 Jindong Gao1 Kun Huang1 Yong Yang1 Xianfeng Hui1 Xinglin He1 Chengfei Li1 Wenxiao Gong1 Yufei Zhang1 Ya Zhao1 Cheng Peng2 Xiaoxiao Gao2 Huanchun Chen1 Zhong Zou1 Zheng-Li Shi2 Meilin Jin1,3,4
Affiliations 4 institutions
  1. National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, People's Republic of China.
  2. CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, People's Republic of China.
  3. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
  4. Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, People's Republic of China.
PMID 32867625 2020 Emerg Microbes Infect eng ppublish
PubMed DOI Browse context

Article

Publication summary

COVID-19 is a new respiratory illness caused by SARS-CoV-2, and has constituted a global public health emergency. Cat is susceptible to SARS-CoV-2. However, the prevalence of SARS-CoV-2 in cats remains largely unknown. Here, we investigated the infection of SARS-CoV-2 in cats during COVID-19 outbreak in Wuhan by serological detection methods. A cohort of serum samples were collected from cats in Wuhan, including 102 sampled after COVID-19 outbreak, and 39 prior to the outbreak. Fifteen sera collected after the outbreak were positive for the receptor binding domain (RBD) of SARS-CoV-2 by indirect enzyme linked immunosorbent assay (ELISA). Among them, 11 had SARS-CoV-2 neutralizing antibodies with a titer ranging from 1/20 to 1/1080. No serological cross-reactivity was detected between SARS-CoV-2 and type I or II feline infectious peritonitis virus (FIPV). In addition, we continuously monitored serum antibody dynamics of two positive cats every 10 days over 130 days. Their serum antibodies reached the peak at 10 days after first sampling, and declined to the limit of detection within 110 days. Our data demonstrated that SARS-CoV-2 has infected cats in Wuhan during the outbreak and described serum antibody dynamics in cats, providing an important reference for clinical treatment and prevention of COVID-19.

cats COVID-19 SARS-CoV-2 serological investigation serum antibody dynamic Animals Antibodies, Neutralizing Antibodies, Viral Betacoronavirus Cats China Coronavirus Infections Coronavirus Nucleocapsid Proteins Coronavirus, Feline COVID-19 Cross Reactions Enzyme-Linked Immunosorbent Assay Immunoglobulin G

Structured evidence records

Evidence records

2 total
Serological Evidence 1 records
Serological Evidence Extraction confidence 1.00
Key finding

Cats in Wuhan had antibodies and neutralizing antibodies to SARS-CoV-2, indicating infection during the COVID-19 outbreak.

Virus
SARS-CoV-2
Location
Not specified
Supporting text

Fifteen sera collected after the outbreak were positive for the receptor binding domain (RBD) of SARS-CoV-2 by indirect enzyme linked immunosorbent assay (ELISA). Among them, 11 had SARS-CoV-2 neutralizing antibodies with a titer ranging from 1/20 to 1/1080.

Method
enzyme linked immunosorbent assay; neutralization test
Sample type
serum
Spillover Event 1 records
Spillover Event Extraction confidence 0.90
Key finding

Cats in Wuhan were infected with SARS-CoV-2 during the COVID-19 outbreak, consistent with human-to-cat spillback.

Virus
SARS-CoV-2
Location
China Wuhan, China
Supporting text

Our data demonstrated that SARS-CoV-2 has infected cats in Wuhan during the outbreak.

Method
enzyme linked immunosorbent assay; neutralization assay
Study design
serological survey
Transmission direction
human-to-animal
Geographic raw
Wuhan
Country inferred
China