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Serologic Screening of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Cats and Dogs during First Coronavirus Disease Wave, the Netherlands.

Shan Zhao Nancy Schuurman Wentao Li Chunyan Wang Lidwien A M Smit Els M Broens Jaap A Wagenaar Frank J M van Kuppeveld Berend-Jan Bosch Herman Egberink
PMID 33900184 2021 Emerg Infect Dis eng ppublish
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Article

Publication summary

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can infect many animal species, including minks, cats, and dogs. To gain insights into SARS-CoV-2 infections in cats and dogs, we developed and validated a set of serologic assays, including ELISA and virus neutralization. Evaluation of samples from animals before they acquired coronavirus disease and samples from cats roaming SARS-CoV-2-positive mink farms confirmed the suitability of these assays for specific antibody detection. Furthermore, our findings exclude SARS-CoV-2 nucleocapsid protein as an antigen for serologic screening of cat and dog samples. We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.

cats coronavirus disease coronaviruses COVID-19 dogs ELISA nucleocapsid protein receptor-binding domain respiratory infections SARS-CoV-2 serologic screening severe acute respiratory syndrome coronavirus 2 the Netherlands virus neutralization viruses zoonoses COVID-19 SARS-CoV-2

Structured evidence records

Evidence records

4 total
Serological Evidence 2 records
Serological Evidence Extraction confidence 1.00
Key finding

SARS-CoV-2 antibodies were detected in 0.4% of cats and 0.2% of dogs tested in the Netherlands, indicating low seroprevalence during the first COVID-19 wave.

Virus
SARS-CoV-2
Location
Not specified
Supporting text

We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive.

Method
ELISA; virus neutralization
Sample type
serum
Serological Evidence Extraction confidence 1.00
Key finding

SARS-CoV-2 antibodies were detected in 0.2% of dogs tested in the Netherlands, indicating low seroprevalence during the first COVID-19 wave.

Virus
SARS-CoV-2
Location
Not specified
Supporting text

We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive.

Method
ELISA; virus neutralization
Sample type
serum
Zoonotic Surveillance 2 records
Zoonotic Surveillance Extraction confidence 0.90
Key finding

Serologic surveillance of domestic cats and dogs in the Netherlands found low seroprevalence for SARS-CoV-2.

Virus
SARS-CoV-2
Location
Netherlands Netherlands
Supporting text

We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.

Method
serology; ELISA; virus neutralization
Sample type
serum
Geographic raw
the Netherlands
Country inferred
Netherlands
Zoonotic Surveillance Extraction confidence 0.90
Key finding

Serologic surveillance of domestic dogs in the Netherlands found low seroprevalence for SARS-CoV-2.

Virus
SARS-CoV-2
Location
Netherlands Netherlands
Supporting text

We analyzed 500 serum samples from domestic cats and dogs in the Netherlands during April-May 2020. We showed 0.4% of cats and 0.2% of dogs were seropositive. Although seroprevalence in cats and dogs that had unknown SARS-CoV-2 exposure was low during the first coronavirus disease wave, our data stress the need for development of continuous serosurveillance for SARS-CoV-2 in these 2 animal species.

Method
serology; ELISA; virus neutralization
Sample type
serum
Geographic raw
the Netherlands
Country inferred
Netherlands