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Publication summary
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global pandemic with significant human impact and zoonotic concerns. In North America, white-tailed deer (<i>Odocoileus virginianus</i>) show high prevalence of infection and viral mutations, raising concerns about novel variants and reverse zoonosis. The World Organisation for Animal Health (WOAH) stated that cervids could become silent reservoirs, urging global surveillance. In Europe, studies have not found significant spill-over into wild deer populations, with negative results in red, roe, fallow, and other deer species across Poland, Germany, Austria, and the UK. However, seropositivity was recently detected in urban fallow deer in Ireland. To assess SARS-CoV-2 presence in Swedish roe deer (<i>Capreolus capreolus</i>), retropharyngeal lymph nodes from 110 individuals (2016-2022) were tested for viral nucleic acid. All samples were negative, suggesting limited or no spill-over in Sweden. These findings align with broader European data but contrast with North American trends, highlighting the importance of continued wildlife monitoring.