OmniVira

C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans.

Carmen P Alvarez1 Fátima Lasala Jaime Carrillo Oscar Muñiz Angel L Corbí Rafael Delgado
Affiliations 1 institutions
  1. Laboratory of Molecular Microbiology, Dept. of Microbiology, Hospital 12 de Octubre, 28041 Madrid, Spain.
PMID12050398 DOI10.1128/jvi.76.13.6841-6844.2002 Year2002 Journal Journal of virology J Virol Languageeng Statusppublish

Abstract

Ebola virus is a highly lethal pathogen responsible for several outbreaks of hemorrhagic fever. Here we show that the primate lentiviral binding C-type lectins DC-SIGN and L-SIGN act as cofactors for cellular entry by Ebola virus. Furthermore, DC-SIGN on the surface of dendritic cells is able to function as a trans receptor, binding Ebola virus-pseudotyped lentiviral particles and transmitting infection to susceptible cells. Our data underscore a role for DC-SIGN and L-SIGN in the infective process and pathogenicity of Ebola virus infection.

Supporting text Virus Host Location
Cell Adhesion Molecules 1 Lectins, C-Type 2 DC-Specific ICAM-3 Grabbing Nonintegrin 1 Dendritic Cells 6 Ebolavirus 31 Humans 1440 Jurkat Cells 1 Lectins 4 Monocytes 3 Receptors, Antigen 1 Receptors, Cell Surface 28 Receptors, Virus 204 Tumor Cells, Cultured 1 Virion 8 CLEC4M protein, human 1

Evidence records

2 total
Functional Mechanism
2 records · 1 evidence types
Evidence type Receptor Usage
2 records
OVE130 Receptor Usage
Key finding

C-type lectins DC-SIGN and L-SIGN act as cellular cofactors mediating Ebola virus entry.

Virus
Ebola virus
Host
primate cells Primates Experimental system
Location
Not specified
Supporting text

Here we show that the primate lentiviral binding C-type lectins DC-SIGN and L-SIGN act as cofactors for cellular entry by Ebola virus.

Method
cell entry assay | receptor binding analysis
Receptors
DC-SIGN | L-SIGN
Host factors
C-type lectins
OVE131 Receptor Usage
Key finding

DC-SIGN on dendritic cells functions as a trans receptor for Ebola virus-pseudotyped particles, mediating binding and transmission to susceptible cells.

Virus
Ebola virus
Host
dendritic cells Experimental system
Location
Not specified
Supporting text

DC-SIGN on the surface of dendritic cells is able to function as a trans receptor, binding Ebola virus-pseudotyped lentiviral particles and transmitting infection to susceptible cells.

Method
pseudovirus binding assay | trans infection experiment
Receptors
DC-SIGN