OmniVira

Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China.

Y Guan1 B J Zheng Y Q He X L Liu Z X Zhuang C L Cheung S W Luo P H Li L J Zhang Y J Guan K M Butt K L Wong K W Chan W Lim K F Shortridge K Y Yuen J S M Peiris L L M Poon
Affiliations 1 institutions
  1. Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China. [email protected]
PMID12958366 DOI10.1126/science.1087139 Year2003 Journal Science (New York, N.Y.) Science Languageeng Statusppublish

Abstract

A novel coronavirus (SCoV) is the etiological agent of severe acute respiratory syndrome (SARS). SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market. All the animal isolates retain a 29-nucleotide sequence that is not found in most human isolates. The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known.

Supporting text Virus Host Location
Amino Acid Sequence 128 Animals 1948 Animals, Wild 187 Antibodies, Viral 212 Blotting, Western 6 Carnivora 6 China 229 Coronavirus 92 Coronavirus Infections 171 Disease Reservoirs 149 Feces 113 Genome, Viral 317 Humans 1440 Membrane Glycoproteins 26 Molecular Sequence Data 160 Neutralization Tests 31 Nose 8 Open Reading Frames 25 Phylogeny 805 Polymorphism, Genetic 9 Reverse Transcriptase Polymerase Chain Reaction 35 Sequence Deletion 8 Sequence Homology, Nucleic Acid 9 Severe acute respiratory syndrome-related coronavirus 78

Evidence records

5 total
Zoonotic Surveillance
4 records · 2 evidence types
Evidence type Virus Isolation
1 records
Evidence type Viral Detection
3 records
OVE11380 Viral Detection
Key finding

Evidence of SCoV-like virus infection was detected in a raccoon dog (Nyctereutes procyonoides) at a live-animal market in Guangdong, China.

Virus
SARS-related coronavirus
Host
raccoon dog Nyctereutes procyonoides Natural host
Location
China
Supporting text

SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market.

Method
virus detection | molecular analysis
Sample type
market animal specimen
Geographic raw
Guangdong, China
Country inferred
CHN
OVE11381 Viral Detection
Key finding

Evidence of SCoV-like virus infection was detected in humans working at the same market in Guangdong, China.

Virus
SARS-related coronavirus
Host
human Homo sapiens Natural host
Location
China
Supporting text

SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market.

Method
virus detection | molecular analysis
Sample type
human specimen
Geographic raw
Guangdong, China
Country inferred
CHN
OVE11383 Viral Detection
Key finding

SCoV-like viruses were isolated from civets and detected in raccoon dogs at a live-animal market in Guangdong, China, indicating interspecies transmission among wild mammals.

Virus
SARS-related coronavirus
Host
masked palm civet Paguma larvata Natural host raccoon dog Nyctereutes procyonoides Natural host
Location
China
Supporting text

SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market.

Method
virus isolation | detection
Geographic raw
Guangdong, China
Genomic Evolution
1 records · 1 evidence types
Evidence type Phylogenetic Evolution
1 records
OVE11382 Phylogenetic Evolution
Key finding

Animal SARS-CoV-like isolates from Himalayan palm civets retain a 29-nucleotide sequence absent in most human SARS-CoV isolates, indicating genomic divergence between animal and human lineages.

Virus
SARS-related coronavirus Severe Acute Respiratory Syndrome Coronavirus
Host
masked palm civet Paguma larvata Natural host human Homo sapiens Natural host
Location
Not specified
Supporting text

All the animal isolates retain a 29-nucleotide sequence that is not found in most human isolates.

Analysis methods
comparative genomic analysis