Literature detail

Identification of a severe acute respiratory syndrome coronavirus-like virus in a leaf-nosed bat in Nigeria.

Phenix-Lan Quan¹ Cadhla Firth Craig Street Jose A Henriquez Alexandra Petrosov Alla Tashmukhamedova Stephen K Hutchison Michael Egholm Modupe O V Osinubi Michael Niezgoda Albert B Ogunkoya Thomas Briese Charles E Rupprecht W Ian Lipkin

Affiliations 1 institutions

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. [email protected]

PMID 21063474 2010 mBio eng epublish

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Article

Publication summary

Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.

Animals Chiroptera Disease Reservoirs Humans Molecular Sequence Data Nigeria Phylogeny Severe Acute Respiratory Syndrome Severe acute respiratory syndrome-related coronavirus

Structured evidence records

Evidence records

3 total

Genomic Evolution 1 records

Genomic Evolution Extraction confidence 0.90

Key finding

Genomic sequencing and phylogenetic analyses identified a novel SARS-CoV-like virus in a Commerson's leaf-nosed bat in Nigeria that forms a new subgroup within group 2 coronaviruses.

Virus

Host

Location

Supporting text

Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.

Genes or proteins: M; N; open reading frames; stem-loop II motifs
Analysis methods: high-throughput pyrosequencing; genomic sequencing; phylogenetic analysis

Reservoir Ecology 1 records

Reservoir Ecology Extraction confidence 0.95

Key finding

A SARS-CoV–like coronavirus was detected in Commerson's leaf-nosed bats from Nigerian caves frequented by humans, indicating bats as potential reservoirs and an ecological interface for spillover.

Virus

Host

Location

Supporting text

Coronavirus consensus PCR and pyrosequencing revealed SARS-CoV-related sequences in a Commerson's leaf-nosed bat (Hipposideros commersoni) collected in caves in Nigeria that are frequented by humans.

Method: pathogen discovery survey; coronavirus consensus PCR; high-throughput pyrosequencing
Sample type: gastrointestinal tissue
Geographic raw: caves in Nigeria
Country inferred: Nigeria

Zoonotic Surveillance 1 records

Zoonotic Surveillance Extraction confidence 0.95

Key finding

Surveillance of bats in human-frequented caves in Nigeria detected SARS-like coronavirus sequences in a Commerson's leaf-nosed bat.

Virus

Host

Location

Supporting text

We surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni).

Method: Coronavirus consensus PCR; high-throughput pyrosequencing
Sample type: gastrointestinal tissue
Geographic raw: Nigeria
Country inferred: Nigeria

Citation context

References

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Evidence overview

Evidence 3

Types 3

Virus 1

Host 1

Evidence types

Genomic Evolution 1 Reservoir Ecology 1 Zoonotic Surveillance 1

Linked entities

Viruses

Hosts

Locations

Publication metadata

Journal: mBio
Volume / Issue / Pages: 1 / 4 / -
ISSN: 2150-7511
Journal country: United States
DOI: 10.1128/mBio.00208-10