Identification of a severe acute respiratory syndrome coronavirus-like virus in a leaf-nosed bat in Nigeria.

Phenix-Lan Quan¹ Cadhla Firth Craig Street Jose A Henriquez Alexandra Petrosov Alla Tashmukhamedova Stephen K Hutchison Michael Egholm Modupe O V Osinubi Michael Niezgoda Albert B Ogunkoya Thomas Briese Charles E Rupprecht W Ian Lipkin

Affiliations 1 institutions

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, USA. [email protected]

PMID21063474 DOI10.1128/mBio.00208-10 Year2010 Journal mBio Languageeng Statusepublish

Abstract

Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.

Supporting text Virus Host Location

Animals 1948 Chiroptera 371 Disease Reservoirs 149 Humans 1440 Molecular Sequence Data 160 Nigeria 19 Phylogeny 805 Severe Acute Respiratory Syndrome 22 Severe acute respiratory syndrome-related coronavirus 78

Evidence records

2 total

Zoonotic Surveillance

1 records · 1 evidence types

Evidence type Viral Detection

1 records

OVE887 Viral Detection

Key finding

Coronavirus sequences related to SARS-CoV were detected in a Commerson's leaf-nosed bat from Nigeria using PCR and pyrosequencing.

Virus

Host

Location

Supporting text

In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni).

Method: consensus PCR | high-throughput pyrosequencing
Sample type: gastrointestinal tissue
Geographic raw: Nigeria
Country inferred: NGA

Genomic Evolution

1 records · 1 evidence types

Evidence type Phylogenetic Evolution

1 records

OVE888 Phylogenetic Evolution

Key finding

Phylogenetic analysis showed that the coronavirus detected in a Commerson's leaf-nosed bat from Nigeria represents a new subgroup within group 2 coronaviruses, distinct from subgroup 2b that includes SARS-CoV.

Virus

Host

Location

Supporting text

Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs.

Genes or proteins: M gene | N gene
Analysis methods: phylogenetic analysis | genomic sequencing

References

54 references

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Evidence overview

Evidence 2

Categories 2

Types 2

Virus 2

Host 1

Country 1

Host context

Natural host 2

Evidence categories

Linked entities

Viruses

Hosts

Commerson's roundleaf bat Macronycteris commersonii

Locations

Publication metadata

Journal: mBio
Volume / Issue / Pages: 1 / 4 / -
ISSN: 2150-7511
Journal country: United States
DOI: 10.1128/mBio.00208-10