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Literature detail

Highly diversified coronaviruses in neotropical bats.

Victor Max Corman1 Andrea Rasche2,1 Thierno Diawo Diallo1 Veronika M Cottontail3 Andreas Stöcker4 Breno Frederico de Carvalho Dominguez Souza4 Jefferson Ivan Corrêa4 Aroldo José Borges Carneiro5 Carlos Roberto Franke5 Martina Nagy6 Markus Metz7 Mirjam Knörnschild3 Elisabeth K V Kalko8,3 Simon J Ghanem9 Karen D Sibaja Morales10 Egoitz Salsamendi11,3 Manuel Spínola10 Georg Herrler2 Christian C Voigt9 Marco Tschapka8,3 Christian Drosten1 Jan Felix Drexler1
Affiliations 11 institutions
  1. Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.
  2. University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
  3. Institute of Experimental Ecology, University of Ulm, Ulm, Germany.
  4. Infectious Diseases Research Laboratory, University Hospital Professor Edgard Santos, Federal University of Bahia, Salvador, Brazil.
  5. School of Veterinary Medicine, Federal University of Bahia, Salvador, Brazil.
  6. Museum für Naturkunde, Leibniz Institute for Research on Evolution and Biodiversity, Berlin, Germany.
  7. Fondazione Edmund Mach, Research and Innovation Centre, Department of Biodiversity and Molecular Ecology, S. Michele all'Adige, Italy.
  8. Smithsonian Tropical Research Institute, Balboa, Panama.
  9. Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.
  10. Instituto Internacional en Conservación y Manejo de Vida Silvestre, Universidad Nacional, Heredia, Costa Rica.
  11. Department of Zoology and Animal Cell Biology, University of the Basque Country, Bilbao, The Basque Country.
PMID 23761408 2013 J Gen Virol eng ppublish
PubMed DOI Browse context

Article

Publication summary

Bats host a broad diversity of coronaviruses (CoVs), including close relatives of human pathogens. There is only limited data on neotropical bat CoVs. We analysed faecal, blood and intestine specimens from 1562 bats sampled in Costa Rica, Panama, Ecuador and Brazil for CoVs by broad-range PCR. CoV RNA was detected in 50 bats representing nine different species, both frugivorous and insectivorous. These bat CoVs were unrelated to known human or animal pathogens, indicating an absence of recent zoonotic spill-over events. Based on RNA-dependent RNA polymerase (RdRp)-based grouping units (RGUs) as a surrogate for CoV species identification, the 50 viruses represented five different alphacoronavirus RGUs and two betacoronavirus RGUs. Closely related alphacoronaviruses were detected in Carollia perspicillata and C. brevicauda across a geographical distance exceeding 5600 km. Our study expands the knowledge on CoV diversity in neotropical bats and emphasizes the association of distinct CoVs and bat host genera.

Genetic Variation Americas Animals Blood Chiroptera Cluster Analysis Coronavirus Feces Intestines Molecular Sequence Data Phylogeography RNA, Viral RNA-Dependent RNA Polymerase Sequence Analysis, DNA

Structured evidence records

Evidence records

3 total
Genomic Evolution 1 records
Genomic Evolution Extraction confidence 0.90
Key finding

Sequence and grouping analysis of coronavirus RdRp sequences from neotropical bats identified five alphacoronavirus and two betacoronavirus lineages.

Host
Chiroptera
Location
Not specified
Supporting text

Based on RNA-dependent RNA polymerase (RdRp)-based grouping units (RGUs) as a surrogate for CoV species identification, the 50 viruses represented five different alphacoronavirus RGUs and two betacoronavirus RGUs.

Genes or proteins
RNA-dependent RNA polymerase (RdRp)
Analysis methods
sequence analysis; phylogenetic grouping
Reservoir Ecology 1 records
Reservoir Ecology Extraction confidence 0.90
Key finding

Coronaviruses were detected in multiple species of neotropical bats sampled in Costa Rica, Panama, Ecuador, and Brazil, indicating bats as ecological reservoirs of diverse CoVs in these regions.

Host
Chiroptera
Location
Costa Rica Ecuador Brazil Panama
Supporting text

We analysed faecal, blood and intestine specimens from 1562 bats sampled in Costa Rica, Panama, Ecuador and Brazil for CoVs by broad-range PCR. CoV RNA was detected in 50 bats representing nine different species, both frugivorous and insectivorous.

Method
broad-range PCR; field sampling
Sample type
faecal; blood; intestine
Geographic raw
Costa Rica, Panama, Ecuador and Brazil
Zoonotic Surveillance 1 records
Zoonotic Surveillance Extraction confidence 0.98
Key finding

Coronavirus RNA was detected in 50 bats from multiple neotropical species sampled in Costa Rica, Panama, Ecuador, and Brazil, revealing high viral diversity in wild bats.

Host
Chiroptera
Location
Costa Rica Ecuador Brazil Panama
Supporting text

We analysed faecal, blood and intestine specimens from 1562 bats sampled in Costa Rica, Panama, Ecuador and Brazil for CoVs by broad-range PCR.

Method
broad-range PCR
Sample type
faeces; blood; intestine
Geographic raw
Costa Rica, Panama, Ecuador and Brazil