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Literature detail

Novel reassortant H5N5 viruses bind to a human-type receptor as a factor in pandemic risk.

Qunhui Li1 Xuan Wang1 Zhao Gao1 Zhongtao Sun1 Zhu Cui1 Zhiqiang Duan1 Juan Li1 Min Gu1 Xiaoquan Wang2,3 Jiao Hu2,3 Xiaowen Liu2,3 Xiufan Liu2,4
Affiliations 4 institutions
  1. Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.
  2. Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China
  3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China.
  4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China. Electronic address: [email protected].
PMID 25575877 2015 Vet Microbiol eng ppublish
PubMed DOI Browse context

Article

Publication summary

Highly pathogenic avian influenza A(HPAI) H5N1 viruses pose a serious pandemic threat due to their virulence and high mortality in humans, and their increasingly expanding host range and significant ongoing evolution could enhance their human-to-human transmissibility. Recently, various reassortant viruses were detected in different domestic poultry, with the HA gene derived from the A/goose/Guangdong/1/96-like (Gs/GD-like) lineage and the NA gene from influenza viruses of other subtypes. It is reported that some natural reassortant H5N5 highly pathogenic avian influenza viruses were isolated from poultry in China. And their HA genes were belonged to a new clade 2.3.4.4. We evaluated the receptor binding property and transmissibility in guinea pigs of these reassortant H5N5 HPAIVs. The results showed that these viruses bound to both avian-type (α-2,3) and human-type (α-2,6) receptors. In addition, we found that one of these viruses, 031, not only replicated but also transmitted efficiently in guinea pigs. Therefore, such reassortant influenza viruses may pose a pandemic threat.

Avian influenza virus H5N5 Reassortant Receptor binding Transmissibility Animals China Guinea Pigs Influenza A Virus, H5N1 Subtype Influenza in Birds Poultry Poultry Diseases Reassortant Viruses Receptors, Cell Surface

Structured evidence records

Evidence records

3 total
Host Range Experiment 1 records
Host Range Experiment Extraction confidence 0.95
Key finding

Reassortant H5N5 highly pathogenic avian influenza virus strain 031 replicated and transmitted efficiently in guinea pigs and exhibited binding to both avian- and human-type receptors.

Virus
IAV H5N5
Location
Not specified
Supporting text

We evaluated the receptor binding property and transmissibility in guinea pigs of these reassortant H5N5 HPAIVs. The results showed that these viruses bound to both avian-type (α-2,3) and human-type (α-2,6) receptors. In addition, we found that one of these viruses, 031, not only replicated but also transmitted efficiently in guinea pigs.

Method
receptor binding assay; replication assay; transmissibility test
Experimental system
in vivo animal experiment
Receptor Usage 1 records
Receptor Usage Extraction confidence 1.00
Key finding

Reassortant H5N5 HPAI viruses bound to both α-2,3 (avian-type) and α-2,6 (human-type) sialic acid receptors.

Virus
IAV H5N5
Location
Not specified
Supporting text

The results showed that these viruses bound to both avian-type (α-2,3) and human-type (α-2,6) receptors.

Method
receptor binding assay
Receptors
sialic acid (α-2,3 and α-2,6)
Recombination Or Reassortment 1 records
Recombination Or Reassortment Extraction confidence 0.95
Key finding

Natural reassortant H5N5 highly pathogenic avian influenza viruses containing HA from a Gs/GD-like lineage and NA from other subtypes were isolated from poultry in China.

Host
Not specified
Location
Not specified
Supporting text

Recently, various reassortant viruses were detected in different domestic poultry, with the HA gene derived from the A/goose/Guangdong/1/96-like (Gs/GD-like) lineage and the NA gene from influenza viruses of other subtypes. It is reported that some natural reassortant H5N5 highly pathogenic avian influenza viruses were isolated from poultry in China.

Event type
reassortment
Genes or segments
HA; NA