OmniVira

Full genomic characterization and phylogenetic analysis of a zoonotic human G8P[14] rotavirus strain detected in a sample from Guatemala.

Rashi Gautam1 Slavica Mijatovic-Rustempasic2 Sunando Roy2 Mathew D Esona2 Beatriz Lopez3 Yolanda Mencos4 Gloria Rey-Benito5 Michael D Bowen2
Affiliations 5 institutions
  1. Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: [email protected].
  2. Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  3. Center for Health Studies, Universidad del Valle de Guatemala, Guatemala City, Guatemala.
  4. Laboratorio Nacional de Salud, Guatemala City, Guatemala.
  5. Pan American Health Organization, Washington, DC, USA.
PMID25952569 DOI10.1016/j.meegid.2015.05.004 Year2015 Journal Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases Infect Genet Evol Languageeng Statusppublish

Abstract

We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. This strain was designated as RVA/Human-wt/GTM/2009726790/2009/G8P[14], with a genomic constellation of G8-P[14]-I2-R2-C2-M2-A13-N2-T6-E2-H3. The VP4 gene occupied lineage VII within the P[14] genotype. Phylogenetic analysis of each genome segment revealed close relatedness to several zoonotic simian, guanaco and bovine strains. Our findings suggest that strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] is an example of a direct zoonotic transmission event. The results of this study reinforce the potential role of interspecies transmission and reassortment in generating novel and rare rotavirus strains which infect humans.

Supporting text Virus Host Location
G8P[14] 2 Interspecies transmission 51 Reassortant 13 Rotavirus 66 Genome, Viral 317 Genomics 32 Phylogeny 805 Animals 1948 Genes, Viral 37 Guatemala 2 Humans 1440 Open Reading Frames 25 Rotavirus 65 Rotavirus Infections 61 Zoonoses 397

Evidence records

4 total
Zoonotic Surveillance
2 records · 1 evidence types
Evidence type Viral Detection
2 records
OVE2028 Viral Detection
Key finding

A human G8P[14] rotavirus strain was detected in a stool sample from Guatemala during routine rotavirus surveillance.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Guatemala
Supporting text

We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance.

Method
genomic characterization | rotavirus surveillance
Sample type
stool sample
Geographic raw
Guatemala
Country inferred
GTM
OVE2030 Viral Detection
Key finding

A human G8P[14] rotavirus strain detected in a stool sample from Guatemala is considered an example of a direct zoonotic transmission event.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Guatemala
Supporting text

We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. Our findings suggest that strain RVA/Human-wt/GTM/2009726790/2009/G8P[14] is an example of a direct zoonotic transmission event.

Method
genomic characterization | phylogenetic analysis
Geographic raw
Guatemala
Genomic Evolution
2 records · 2 evidence types
Evidence type Recombination or Reassortment
1 records
OVE2031 Recombination or Reassortment
Key finding

A rare human G8P[14] rotavirus strain from Guatemala shows evidence of reassortment among zoonotic simian, guanaco, and bovine lineages, contributing to its emergence in humans.

Virus
Rotavirus A
Host
Not specified
Location
Not specified
Supporting text

The results of this study reinforce the potential role of interspecies transmission and reassortment in generating novel and rare rotavirus strains which infect humans.

Event type
reassortment
Evidence type Phylogenetic Evolution
1 records
OVE2029 Phylogenetic Evolution
Key finding

Phylogenetic analysis showed that the human G8P[14] rotavirus genome segments are closely related to simian, guanaco, and bovine strains, indicating zoonotic evolutionary origin.

Virus
Rotavirus A
Host
Simiiformes Natural host guanaco Lama guanicoe Natural host domestic cattle Bos taurus Natural host human Homo sapiens Natural host
Location
Not specified
Supporting text

We report the genomic characterization of a rare human G8P[14] rotavirus strain, identified in a stool sample from Guatemala (GTM) during routine rotavirus surveillance. Phylogenetic analysis of each genome segment revealed close relatedness to several zoonotic simian, guanaco and bovine strains.

Genes or proteins
genome segments | VP4 gene
Analysis methods
phylogenetic analysis | genomic characterization