Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission.
An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea. In this study, we sequenced and characterized the complete genome of strain KKL-117. On full-genomic analysis, strain KKL-117 was found to have the following genotype constellation: G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The non-G/P genotype constellation of this strain (I5-R1-C1-M1-A8-N1-T1-E1-H1) is commonly shared with rotavirus strains from pigs. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin. Our observations provide important insights into the dynamic interactions between human and porcine rotavirus strains.
Complete genome sequencing and phylogenetic analysis of the human G9P[23] rotavirus strain KKL-117 revealed that all 11 genes were derived from porcine rotaviruses, supporting a porcine origin of this human strain.
We sequenced and characterized the complete genome of strain KKL-117. On full-genomic analysis, strain KKL-117 was found to have the following genotype constellation: G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin.
Analysis methods
complete genome sequencing; phylogenetic analysis
Recombination Or Reassortment1 records
Recombination Or ReassortmentExtraction confidence 0.90
Key finding
Human G9P[23] rotavirus strain KKL-117 is a reassortant containing porcine-origin genome segments, consistent with porcine-to-human interspecies transmission.
On full-genomic analysis, strain KKL-117 was found to have the genotype constellation G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1, with the non-G/P genotype constellation commonly shared with rotavirus strains from pigs. Phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin.
Event type
reassortment
Genes or segments
I5; R1; C1; M1; A8; N1; T1; E1; H1
Spillover Event1 records
Spillover EventExtraction confidence 0.98
Key finding
Porcine-origin rotavirus strain G9P[23] was detected in a human child, showing porcine-to-human spillover in Thailand.
An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea... The non-G/P genotype constellation of this strain is commonly shared with rotavirus strains from pigs... providing evidence for porcine-to-human interspecies transmission.
