OmniVira

Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission.

Satoshi Komoto1 Ratana Tacharoenmuang2 Ratigorn Guntapong2 Tomihiko Ide1 Phakapun Sinchai2 Sompong Upachai2 Saori Fukuda1 Tetsushi Yoshikawa3 Piyanit Tharmaphornpilas4 Somchai Sangkitporn2 Koki Taniguchi1
Affiliations 4 institutions
  1. Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
  2. Department of Medical Sciences, National Institute of Health, Ministry of Public Health, Nonthaburi 11000, Thailand.
  3. Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
  4. Department of Disease Control, Ministry of Public Health, Nonthaburi 11000, Thailand.
PMID28382902 DOI10.1099/jgv.0.000722 Year2017 Journal The Journal of general virology J Gen Virol Languageeng Statusppublish

Abstract

An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea. In this study, we sequenced and characterized the complete genome of strain KKL-117. On full-genomic analysis, strain KKL-117 was found to have the following genotype constellation: G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The non-G/P genotype constellation of this strain (I5-R1-C1-M1-A8-N1-T1-E1-H1) is commonly shared with rotavirus strains from pigs. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin. Our observations provide important insights into the dynamic interactions between human and porcine rotavirus strains.

Supporting text Virus Host Location
Genotype 137 Animals 1948 Cluster Analysis 31 Diarrhea 29 Feces 113 Genome, Viral 317 Humans 1440 Infant 26 Phylogeny 805 Rotavirus 65 Rotavirus Infections 61 Sequence Analysis, DNA 113 Sequence Homology 21 Swine 258 Thailand 21 Zoonoses 397

Evidence records

3 total
Zoonotic Surveillance
1 records · 1 evidence types
Evidence type Viral Detection
1 records
OVE2584 Viral Detection
Key finding

A rotavirus strain designated RVA/Human-wt/THA/KKL-117/2014/G9P[23] was detected in a stool specimen from a child with severe diarrhoea in Thailand.

Virus
Rotavirus A Rotavirus
Host
human Homo sapiens Natural host
Location
Thailand
Supporting text

Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission. An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea.

Method
viral genome sequencing | genotype identification
Sample type
stool specimen
Geographic raw
Thailand
Country inferred
THA
Genomic Evolution
2 records · 1 evidence types
Evidence type Phylogenetic Evolution
2 records
OVE2586 Phylogenetic Evolution
Key finding

Genomic and phylogenetic analyses demonstrated porcine-to-human spillover of rotavirus strain KKL-117 (G9P[23]) in a child with diarrhoea in Thailand.

Virus
Rotavirus A
Host
pig Sus scrofa Natural host human Homo sapiens Natural host
Location
Thailand
Supporting text

Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission.

Analysis methods
full-genome sequencing | phylogenetic analysis
OVE2585 Phylogenetic Evolution
Key finding

Phylogenetic analysis demonstrated that rotavirus strain KKL-117 had gene sequences of porcine origin, indicating porcine-to-human evolutionary transmission.

Virus
Rotavirus A Rotavirus
Host
pig Sus scrofa Natural host
Location
Not specified
Supporting text

The non-G/P genotype constellation of this strain (I5-R1-C1-M1-A8-N1-T1-E1-H1) is commonly shared with rotavirus strains from pigs. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin.

Genes or proteins
11 genes | complete genome
Analysis methods
phylogenetic analysis | full-genomic analysis