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Literature detail

Detection of an animal-derived G4P[6] group A rotavirus strain in a symptomatic child, in Italy.

Giovanni Ianiro1 Roberto Micolano2 Michela Conte2 Michele Labianca3 Gabriele Vaccari2 Marina Monini2
Affiliations 3 institutions
  1. Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy. Electronic address: [email protected].
  2. Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
  3. Unit of Hygiene, Epidemiology and Public Health, ASL-Potenza, Villa D'Agri (PZ), Italy.
PMID 30423360 2019 Virus Res eng ppublish
PubMed DOI Browse context

Article

Publication summary

During 2017, a G4P[6] group A rotavirus strain was identified in the feces of an Italian child hospitalized for acute gastroenteritis in Southern Italy. Nucleotide sequencing of the 11 genomic segments, revealed the G4-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analyses of the gene segments investigated revealed high nucleotide sequence identities with G4P[6] RVA strains detected previously in pigs and in humans. The human strains related to the Italian G4P[6] were mainly reported from Asia, and were detected after an inter-species transmission event from swine. This study reports the genotyping and phylogenetic analysis of a G4P[6] RVA strain presenting a genomic constellation never detected before in Italy. In addition, this strain was able to cause AGE symptoms in a healthy child, successively hospitalized. The molecular characterization suggested zoonotic origin and inter-species transmission of this strain from swine, living open the possibility of its importation from abroad.

Diarrhea G4P[6] Human Italy Rotavirus Swine Genotype Animals Child Feces Gastroenteritis Humans Italy Phylogeny RNA, Viral Rotavirus Rotavirus Infections Sequence Analysis, DNA

Structured evidence records

Evidence records

2 total
Genomic Evolution 1 records
Genomic Evolution Extraction confidence 0.90
Key finding

Genomic sequencing and phylogenetic analysis showed that the G4P[6] rotavirus strain infecting a child in Italy was closely related to swine strains, supporting a zoonotic origin via interspecies transmission.

Virus
RVA
Location
Not specified
Supporting text

Nucleotide sequencing of the 11 genomic segments revealed the G4-P[6]-I1-R1-C1-M1-A1-N1-T1-E1-H1 genotype constellation. Phylogenetic analyses of the gene segments investigated revealed high nucleotide sequence identities with G4P[6] RVA strains detected previously in pigs and in humans. The molecular characterization suggested zoonotic origin and inter-species transmission of this strain from swine.

Genes or proteins
11 genomic segments
Analysis methods
nucleotide sequencing; phylogenetic analysis
Spillover Event 1 records
Spillover Event Extraction confidence 0.95
Key finding

A bovine-derived G4P[6] rotavirus with swine-like genetic features caused infection in a human child, consistent with swine-to-human spillover in Italy.

Virus
RVA
Location
Italy Southern Italy, Italy
Supporting text

During 2017, a G4P[6] group A rotavirus strain was identified in the feces of an Italian child hospitalized for acute gastroenteritis in Southern Italy... The molecular characterization suggested zoonotic origin and inter-species transmission of this strain from swine.

Method
nucleotide sequencing; phylogenetic analysis; genotyping
Study design
genomic surveillance
Transmission direction
animal-to-human
Geographic raw
Southern Italy
Country inferred
Italy