Severe Acute Respiratory Syndrome Coronavirus 2 in Farmed Mink (Neovison vison), Poland.

Lukasz Rabalski Maciej Kosinski Teemu Smura Kirsi Aaltonen Ravi Kant Tarja Sironen Bogusław Szewczyk Maciej Grzybek

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent of coronavirus disease and has been spreading worldwide since December 2019. The virus can infect different animal species under experimental conditions, and mink on fur farms in Europe and other areas are susceptible to SARS-CoV-2 infection. We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. Using reverse transcription PCR, antigen detection, and next-generation sequencing, we confirmed that 15 animals were positive for SARS-CoV-2. We verified this finding by sequencing full viral genomes and confirmed a virus variant that has sporadic mutations through the full genome sequence in the spike protein (G75V and C1247F). We were unable to find other SARS-CoV-2 sequences simultaneously containing these 2 mutations. Country-scale monitoring by veterinary inspection should be implemented to detect SARS-CoV-2 in other mink farms.

Supporting text Virus Host Location
coronavirus disease 15 coronaviruses 32 COVID-19 467 detection 3 mink 58 molecular characterization 7 Neovison vison 2 Poland 9 respiratory infections 30 SARS-CoV-2 550 severe acute respiratory syndrome coronavirus 2 20 spillover 105 transmission 55 viruses 117 zoonoses 477 COVID-19 425 Mink 48 Animals 1948 Farms 30 Humans 1440 Poland 9 SARS-CoV-2 453

Evidence records

2 total
Zoonotic Surveillance
1 records · 1 evidence types
Evidence type
1 records
OVE5084
Key finding

SARS-CoV-2 RNA and antigen were detected in 15 mink from a farm in northern Poland using RT-PCR and antigen detection methods.

Virus
Host
Location
Supporting text

We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. Using reverse transcription PCR, antigen detection, and next-generation sequencing, we confirmed that 15 animals were positive for SARS-CoV-2.

Method
reverse transcription PCR | antigen detection | next-generation sequencing
Sample type
farm surveillance specimens
Geographic raw
northern Poland
Country inferred
POL
Genomic Evolution
1 records · 1 evidence types
Evidence type
1 records
OVE5085
Key finding

Full-genome sequencing of SARS-CoV-2 from farmed mink in Poland revealed a variant with spike mutations G75V and C1247F not found in other sequences.

Virus
Host
Location
Not specified
Supporting text

We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. We verified this finding by sequencing full viral genomes and confirmed a virus variant that has sporadic mutations through the full genome sequence in the spike protein (G75V and C1247F).

Genes or proteins
spike protein
Analysis methods
full-genome sequencing