Literature detail

Severe Acute Respiratory Syndrome Coronavirus 2 in Farmed Mink (Neovison vison), Poland.

Lukasz Rabalski Maciej Kosinski Teemu Smura Kirsi Aaltonen Ravi Kant Tarja Sironen Bogusław Szewczyk Maciej Grzybek
PMID 34423763 2021 Emerg Infect Dis eng ppublish
PubMed DOI Browse context

Article

Publication summary

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent of coronavirus disease and has been spreading worldwide since December 2019. The virus can infect different animal species under experimental conditions, and mink on fur farms in Europe and other areas are susceptible to SARS-CoV-2 infection. We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. Using reverse transcription PCR, antigen detection, and next-generation sequencing, we confirmed that 15 animals were positive for SARS-CoV-2. We verified this finding by sequencing full viral genomes and confirmed a virus variant that has sporadic mutations through the full genome sequence in the spike protein (G75V and C1247F). We were unable to find other SARS-CoV-2 sequences simultaneously containing these 2 mutations. Country-scale monitoring by veterinary inspection should be implemented to detect SARS-CoV-2 in other mink farms.

coronavirus disease coronaviruses COVID-19 detection mink molecular characterization Neovison vison Poland respiratory infections SARS-CoV-2 severe acute respiratory syndrome coronavirus 2 spillover transmission viruses zoonoses COVID-19 Mink Animals

Structured evidence records

Evidence records

3 total
1 records
Extraction confidence 0.85
Key finding

Full-genome sequencing of SARS-CoV-2 from farmed mink in Poland identified a variant with unique spike protein mutations G75V and C1247F not found together in other sequences.

Virus
Location
Not specified
Supporting text

We verified this finding by sequencing full viral genomes and confirmed a virus variant that has sporadic mutations through the full genome sequence in the spike protein (G75V and C1247F). We were unable to find other SARS-CoV-2 sequences simultaneously containing these 2 mutations.

Genes or proteins
spike
Analysis methods
whole-genome sequencing; mutation analysis
1 records
Extraction confidence 0.80
Key finding

SARS-CoV-2 was confirmed in farmed mink in Poland, consistent with human-to-mink spillback of the virus.

Virus
Location
Supporting text

We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. Using reverse transcription PCR, antigen detection, and next-generation sequencing, we confirmed that 15 animals were positive for SARS-CoV-2.

Method
reverse transcription PCR; antigen detection; next-generation sequencing; whole genome sequencing
Study design
field surveillance
Transmission direction
human-to-animal
Geographic raw
Poland
Country inferred
Poland
1 records
Extraction confidence 0.95
Key finding

Surveillance of 91 farmed mink in northern Poland identified 15 SARS-CoV-2–positive animals by RT-PCR, antigen detection, and sequencing.

Virus
Location
Supporting text

We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. Using reverse transcription PCR, antigen detection, and next-generation sequencing, we confirmed that 15 animals were positive for SARS-CoV-2.

Method
reverse transcription PCR; antigen detection; next-generation sequencing
Geographic raw
northern Poland
Country inferred
Poland