OmniVira

Genetic diversity in three bovine-like human G8P[14] and G10P[14] rotaviruses suggests independent interspecies transmission events.

Maria Cristina Medici1 Fabio Tummolo1 Melisa Berenice Bonica1 Elisabeth Heylen2 Mark Zeller2 Adriana Calderaro1 Jelle Matthijnssens2
Affiliations 2 institutions
  1. Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  2. KU Leuven - University of Leuven, Department of Microbiology and Immunology, Laboratory for Clinical and Epidemiological Virology, Rega Institute for Medical Research, B-3000 Leuven, Belgium.
PMID25614586 DOI10.1099/vir.0.000055 Year2015 Journal The Journal of general virology J Gen Virol Languageeng Statusppublish

Abstract

The group A rotavirus (RVA) P[14] genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission. The present study describes the full-length genome analysis of two G8P[14] and one G10P[14] human RVAs detected in Italy. The strains possessed the typical bovine-like I2-R2-C2-M2-A3/A11-N2-T6-E2-H3 genotype constellation. All the segments of the two G8P[14] RVAs were most closely related to bovine(-like) strains but were relatively distant to each other, suggesting two independent interspecies transmission events. Likewise, the G10P[14] RVA gene segments were most similar to bovine(-like) RVAs but distinct from the G8 strains. The history of these strains probably involved the interspecies transmission of these viruses to humans from an as-yet-unidentified animal host, without evidence of reassortment events involving human RVAs. These results reinforce the potential of animal viruses to cross the host-species barrier, causing disease and increased viral genetic diversity in humans.

Supporting text Virus Host Location
Genetic Variation 127 Animals 1948 Child, Preschool 37 Cluster Analysis 31 Genome, Viral 317 Genotype 137 Humans 1440 Infant 26 Italy 20 Molecular Sequence Data 160 Phylogeny 805 RNA, Viral 193 Rotavirus 65 Rotavirus Infections 61 Sequence Analysis, DNA 113 Sequence Homology 21 Zoonoses 397

Evidence records

4 total
Genomic Evolution
4 records · 1 evidence types
Evidence type Phylogenetic Evolution
4 records
OVE1927 Phylogenetic Evolution
Key finding

Phylogenetic analysis showed that human G8P[14] rotavirus strains were most closely related to bovine-like strains but distinct from each other, indicating separate evolutionary lineages derived from bovine rotaviruses.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Not specified
Supporting text

All the segments of the two G8P[14] RVAs were most closely related to bovine(-like) strains but were relatively distant to each other, suggesting two independent interspecies transmission events.

Genes or proteins
genome segments
Analysis methods
phylogenetic analysis | comparative genome analysis
OVE1928 Phylogenetic Evolution
Key finding

Phylogenetic analysis indicated that the human G10P[14] rotavirus strain clustered with bovine-like rotaviruses but was distinct from G8P[14] strains, revealing a separate evolutionary origin.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Not specified
Supporting text

Likewise, the G10P[14] RVA gene segments were most similar to bovine(-like) RVAs but distinct from the G8 strains.

Genes or proteins
gene segments
Analysis methods
phylogenetic analysis | comparative genome analysis
OVE1925 Phylogenetic Evolution
Key finding

Bovine-like G8P[14] rotavirus was transmitted independently from animals to humans in Italy.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Italy
Supporting text

The present study describes the full-length genome analysis of two G8P[14] and one G10P[14] human RVAs detected in Italy. All the segments of the two G8P[14] RVAs were most closely related to bovine(-like) strains but were relatively distant to each other, suggesting two independent interspecies transmission events.

Analysis methods
full-length genome analysis | phylogenetic comparison
OVE1926 Phylogenetic Evolution
Key finding

Bovine-like G10P[14] rotavirus was transmitted from animals to humans in Italy.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Italy
Supporting text

The present study describes the full-length genome analysis of two G8P[14] and one G10P[14] human RVAs detected in Italy. Likewise, the G10P[14] RVA gene segments were most similar to bovine(-like) RVAs but distinct from the G8 strains.

Analysis methods
full-length genome analysis | phylogenetic comparison