Full genomic analyses of human rotavirus G4P[4], G4P[6], G9P[19] and G10P[6] strains from North-eastern India: evidence for interspecies transmission and complex reassortment events.
A Mukherjee1
S Ghosh
P Bagchi
D Dutta
S Chattopadhyay
N Kobayashi
M Chawla-Sarkar
Affiliations1 institutions
Division of Virology, National Institute of Cholera and Enteric Diseases, Scheme XM, Beliaghata, Kolkata, India.
In hospitalized patients with acute gastroenteritis in Manipur, India, four rotavirus strains were found to possess VP7 and/or VP4 genes with porcine or bovine characteristics. Considering the animal-like nature of these strains, the remaining eight gene segments were analysed to decipher their exact origin. Analyses of full genome of these strains exhibited their origin from porcine/bovine rotaviruses. This study suggests single or multiple events of reassortment involving multiple gene segments of more than one host type among the strains and emphasizes the significance of complete genetic characterization of unusual strains in regions with high incidence and mortality rates.
Human rotavirus strains in Manipur, India, contained gene segments with porcine and bovine characteristics, indicating animal-to-human transmission of rotaviruses.
In hospitalized patients with acute gastroenteritis in Manipur, India, four rotavirus strains were found to possess VP7 and/or VP4 genes with porcine or bovine characteristics.
Method
genome sequencing; phylogenetic analysis
Study design
genomic surveillance
Transmission direction
animal-to-human
Geographic raw
Manipur, India
Country inferred
India
Spillover EventExtraction confidence 0.80
Key finding
Human rotavirus strains in Manipur, India, contained gene segments with bovine genetic characteristics, consistent with bovine-to-human spillover.
In hospitalized patients with acute gastroenteritis in Manipur, India, four rotavirus strains were found to possess VP7 and/or VP4 genes with porcine or bovine characteristics.
Method
genome sequencing; phylogenetic analysis
Study design
genomic surveillance
Transmission direction
animal-to-human
Geographic raw
Manipur, India
Country inferred
India
Cross Species Transmission1 records
Cross Species TransmissionExtraction confidence 0.95
Key finding
Rotavirus genome analyses revealed gene segments of porcine and bovine origin, indicating cross-species transmission and reassortment among animal rotaviruses.
Four rotavirus strains were found to possess VP7 and/or VP4 genes with porcine or bovine characteristics. Analyses of full genomes of these strains exhibited their origin from porcine/bovine rotaviruses, suggesting single or multiple events of reassortment involving more than one host type among the strains.
Method
full genomic analysis; phylogenetic analysis; reassortment analysis
Study design
phylogenetic analysis
Transmission direction
animal-to-animal
Geographic raw
Manipur, India
Country inferred
India
Genomic Evolution1 records
Genomic EvolutionExtraction confidence 0.85
Key finding
Full-genome analysis indicated that human rotavirus strains have genetic segments originating from porcine and bovine rotaviruses through reassortment.
Analyses of full genome of these strains exhibited their origin from porcine/bovine rotaviruses.
Genes or proteins
VP7; VP4
Analysis methods
full genome analysis; phylogenetic analysis
Recombination Or Reassortment1 records
Recombination Or ReassortmentExtraction confidence 0.95
Key finding
Human rotavirus strains G4P[4], G4P[6], G9P[19], and G10P[6] in Manipur, India, underwent reassortment between porcine and bovine rotaviruses, reflecting interspecies transmission.
Analyses of full genome of these strains exhibited their origin from porcine/bovine rotaviruses. This study suggests single or multiple events of reassortment involving multiple gene segments of more than one host type among the strains.
