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Literature detail

Identical consensus sequence and conserved genomic polymorphism of hepatitis E virus during controlled interspecies transmission.

Jerome Bouquet1 Justine Cheval Sophie Rogée Nicole Pavio Marc Eloit
Affiliations 1 institutions
  1. UMR 1161 Virology, ANSES, Laboratoire de Santé Animale, Maisons-Alfort, France.
PMID 22457521 2012 J Virol eng ppublish
PubMed DOI Browse context

Article

Publication summary

High-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis E virus (HEV) of genotype 3f revealed the same full-length consensus sequence as in the human sample. Twenty-nine percent of polymorphic sites found in HEV from the human sample were conserved throughout the infection of the heterologous host. The interspecies transmission of HEV quasispecies is the result of a genomic negative-selection pressure on random mutations which can be deleterious to the viral population. HEV intrahost nucleotide diversity was found to be in the lower range of other human RNA viruses but correlated with values found for zoonotic viruses. HEV transmission between humans and pigs does not seem to be modulated by host-specific mutations, suggesting that adaptation is mainly regulated by ecological drivers.

Polymorphism, Genetic Animals Consensus Sequence Disease Models, Animal Genome, Viral Hepatitis E Hepatitis E virus Humans Molecular Sequence Data RNA, Viral Sequence Analysis, DNA Swine

Structured evidence records

Evidence records

3 total
Genomic Evolution 1 records
Genomic Evolution Extraction confidence 0.90
Key finding

Experimental transmission of hepatitis E virus genotype 3f from humans to pigs showed identical viral genome sequences and conserved polymorphisms, indicating limited genomic evolution across hosts.

Virus
HEV
Host
Sus scrofa
Location
Not specified
Supporting text

High-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis E virus (HEV) of genotype 3f revealed the same full-length consensus sequence as in the human sample. Twenty-nine percent of polymorphic sites found in HEV from the human sample were conserved throughout the infection of the heterologous host.

Genes or proteins
whole genome
Analysis methods
high-throughput sequencing; sequence analysis
Host Range Experiment 1 records
Host Range Experiment Extraction confidence 0.95
Key finding

Human hepatitis E virus genotype 3f successfully infected and replicated in pigs, maintaining the same consensus sequence as the original human isolate.

Virus
HEV
Location
Not specified
Supporting text

High-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis E virus (HEV) of genotype 3f revealed the same full-length consensus sequence as in the human sample.

Method
experimental infection; high-throughput sequencing
Sample type
bile; feces
Experimental system
in vivo animal experiment
Molecular Adaptation 1 records
Molecular Adaptation Extraction confidence 0.80
Key finding

Human hepatitis E virus genotype 3f transmitted to pigs retained identical genomic consensus sequence, showing absence of host-specific mutations and limited molecular adaptation.

Virus
HEV
Host
Not specified
Location
Not specified
Supporting text

High-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis E virus (HEV) of genotype 3f revealed the same full-length consensus sequence as in the human sample. Twenty-nine percent of polymorphic sites found in HEV from the human sample were conserved throughout the infection of the heterologous host. HEV transmission between humans and pigs does not seem to be modulated by host-specific mutations, suggesting that adaptation is mainly regulated by ecological drivers.

Mechanism types
replication_efficiency; host_specific_mutation