MERS-related CoVs in hedgehogs from Hubei Province, China.

Dan Li¹ Xiao-Qing Gong² Xiao Xiao³ Hui-Ju Han¹ Hao Yu⁴ Ze-Min Li¹ Li-Na Yan¹ Xiao-Lan Gu¹ Shu-Hui Duan¹ Xue-jieYu¹

Affiliations 4 institutions

State Key Laboratory of Virology, School of Health Sciences, Wuhan University, Wuhan, Hubei, China.
Double First-class Construction Office, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Lab Animal Research Center, Hubei University of Chinese Medicine, Wuhan, Hubei, China.
Department of Neuroscience, Cell Biology, and Anatomy, 301 University Blvd, Galveston, TX 77555, United States of America.

PMID34604493 DOI10.1016/j.onehlt.2021.100332 Year2021 Journal One health (Amsterdam, Netherlands) One Health Languageeng Statusppublish

Abstract

The emerging coronavirus diseases such as COVID-19, MERS, and SARS indicated that animal coronaviruses (CoVs) spillover to humans are a huge threat to public health. Therefore, we needed to understand the CoVs carried by various animals. Wild hedgehogs were collected from rural areas in Wuhan and Xianning cities in Hubei Province for analysis of CoVs. PCR results showed that 5 out of 51 (9.8%) hedgehogs (Erinaceus amurensis) were positive to CoVs in Hubei Province with 3 samples from Wuhan City and 2 samples from Xianning City. Phylogenetic analysis based on the partial sequence of RNA-dependent RNA polymerase showed that the CoVs from hedgehogs are classified into Merbecovirus of the genus Betacoronavirus; the hedgehog CoVs formed a phylogenetic sister cluster with human MERS-CoVs and bat MERS-related CoVs. Among the 12 most critical residues of receptor binding domain in MERS-CoV for binding human Dipeptidyl peptidase 4, 3 residuals were conserved between the hedgehog MERS-related CoV obtained in this study and the human MERS-CoV. We concluded that hedgehogs from Hubei Province carried MERS-related CoVs, indicating that hedgehogs might be important in the evolution and transmission of MERS-CoVs, and continuous surveillance of CoVs in hedgehogs was important.

Supporting text Virus Host Location

Betacoronavirus 89 Coronavirus 195 Hedgehogs 8 Merbecovirus 12 MERS-CoVs 1

Evidence records

3 total

Zoonotic Surveillance

2 records · 2 evidence types

Evidence type Viral Detection

1 records

OVE5198 Viral Detection

Key finding

PCR testing detected MERS-related coronaviruses in hedgehogs (Erinaceus amurensis) in Hubei Province, with positive samples from Wuhan and Xianning.

Virus

Host

Location

Supporting text

PCR results showed that 5 out of 51 (9.8%) hedgehogs (Erinaceus amurensis) were positive to CoVs in Hubei Province with 3 samples from Wuhan City and 2 samples from Xianning City.

Method: PCR
Sample type: hedgehog samples
Geographic raw: Wuhan City | Xianning City | Hubei Province

Evidence type Reservoir Ecology

1 records

OVE5201 Reservoir Ecology

Key finding

Hedgehogs from Hubei Province carry MERS-related coronaviruses and may play an ecological role in the evolution and transmission of MERS-CoVs.

Virus

Host

Location

Supporting text

We concluded that hedgehogs from Hubei Province carried MERS-related CoVs, indicating that hedgehogs might be important in the evolution and transmission of MERS-CoVs.

Method: PCR detection | phylogenetic analysis
Geographic raw: Hubei Province

Genomic Evolution

1 records · 1 evidence types

Evidence type Phylogenetic Evolution

1 records

OVE5199 Phylogenetic Evolution

Key finding

Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene showed that hedgehog coronaviruses from Hubei clustered as a sister group to human MERS-CoVs and bat MERS-related CoVs within the Merbecovirus lineage.

Virus

Host

hedgehogs Erinaceidae Natural host

human Homo sapiens Natural host

bats Chiroptera Natural host

Location

Supporting text

Phylogenetic analysis based on the partial sequence of RNA-dependent RNA polymerase showed that the CoVs from hedgehogs are classified into Merbecovirus of the genus Betacoronavirus; the hedgehog CoVs formed a phylogenetic sister cluster with human MERS-CoVs and bat MERS-related CoVs.

Genes or proteins: RNA-dependent RNA polymerase
Analysis methods: phylogenetic analysis

References

49 references

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