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Literature detail

Isolation of Bat Sarbecoviruses, Japan.

Shin Murakami Tomoya Kitamura Hiromichi Matsugo Haruhiko Kamiki Ken Oyabu Wataru Sekine Akiko Takenaka-Uema Yuko Sakai-Tagawa Yoshihiro Kawaoka Taisuke Horimoto
PMID 36417954 2022 Emerg Infect Dis eng ppublish
PubMed DOI Browse context

Article

Publication summary

Surveillance of bat betacoronaviruses is crucial for understanding their spillover potential. We isolated bat sarbecoviruses from Rhinolophus cornutus bats in multiple locations in Japan. These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2, suggesting a narrow host range.

ACE2 angiotensin converting enzyme-2 bat betacoronavirus coronaviruses Japan respiratory infections Rhinolophus sarbecovirus SARS-CoV-2 SARS-related coronavirus severe acute respiratory syndrome coronavirus 2 virus isolation viruses zoonoses Chiroptera Animals Betacoronavirus

Structured evidence records

Evidence records

4 total
Host Range Experiment 2 records
Host Range Experiment Extraction confidence 0.90
Key finding

Bat sarbecoviruses replicated efficiently in cells expressing Rhinolophus cornutus ACE2 but not in human ACE2-expressing cells, demonstrating host range restriction.

Virus
Sarbecovirus
Location
Not specified
Supporting text

These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2, suggesting a narrow host range.

Method
virus isolation; cell-entry assay; replication assay
Experimental system
in vitro cell culture
Host Range Experiment Extraction confidence 0.90
Key finding

Bat sarbecoviruses did not grow in cells expressing human ACE2, indicating limited ability to infect human receptor-bearing cells.

Virus
Sarbecovirus
Location
Not specified
Supporting text

These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2, suggesting a narrow host range.

Method
virus isolation; cell-entry assay; replication assay
Experimental system
in vitro cell culture
Receptor Usage 1 records
Receptor Usage Extraction confidence 0.90
Key finding

Bat sarbecoviruses isolated from Rhinolophus cornutus used R. cornutus ACE2 as a functional receptor but could not utilize human ACE2, demonstrating receptor specificity barriers between bat and human hosts.

Virus
Sarbecovirus
Location
Not specified
Supporting text

These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2, suggesting a narrow host range.

Method
cell entry assay
Receptors
angiotensin converting enzyme-2
Zoonotic Surveillance 1 records
Zoonotic Surveillance Extraction confidence 0.95
Key finding

Bat sarbecoviruses were isolated from Rhinolophus cornutus bats during betacoronavirus surveillance in Japan.

Virus
Sarbecovirus
Location
Japan Japan
Supporting text

Surveillance of bat betacoronaviruses is crucial for understanding their spillover potential. We isolated bat sarbecoviruses from Rhinolophus cornutus bats in multiple locations in Japan.

Method
virus isolation
Geographic raw
Japan
Country inferred
Japan