OmniVira

Isolation of Bat Sarbecoviruses, Japan.

Shin Murakami Tomoya Kitamura Hiromichi Matsugo Haruhiko Kamiki Ken Oyabu Wataru Sekine Akiko Takenaka-Uema Yuko Sakai-Tagawa Yoshihiro Kawaoka Taisuke Horimoto
PMID36417954 DOI10.3201/eid2812.220801 Year2022 Journal Emerging infectious diseases Emerg Infect Dis Languageeng Statusppublish

Abstract

Surveillance of bat betacoronaviruses is crucial for understanding their spillover potential. We isolated bat sarbecoviruses from Rhinolophus cornutus bats in multiple locations in Japan. These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2, suggesting a narrow host range.

Supporting text Virus Host Location
ACE2 54 angiotensin converting enzyme-2 1 bat 54 betacoronavirus 89 coronaviruses 32 Japan 15 respiratory infections 30 Rhinolophus 3 sarbecovirus 19 SARS-CoV-2 550 SARS-related coronavirus 5 severe acute respiratory syndrome coronavirus 2 20 virus isolation 7 viruses 117 zoonoses 477 Chiroptera 371 Animals 1948 Betacoronavirus 78 Host Specificity 132 Humans 1440 Japan 12 Peptidyl-Dipeptidase A 57

Evidence records

2 total
Zoonotic Surveillance
1 records · 1 evidence types
Evidence type Virus Isolation
1 records
Functional Mechanism
1 records · 1 evidence types
Evidence type Receptor Usage
1 records
OVE6539 Receptor Usage
Key finding

Bat sarbecoviruses use Rhinolophus cornutus ACE2 but not human ACE2 as the entry receptor.

Virus
Sarbecovirus
Host
Little Japanese Horseshoe Bat Rhinolophus cornutus Experimental system human Homo sapiens Experimental system
Location
Not specified
Supporting text

These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2.

Method
cell culture infection assay | receptor expression experiment
Receptors
R. cornutus angiotensin converting enzyme-2 | human angiotensin converting enzyme-2