Isolation of Bat Sarbecoviruses, Japan.

Shin Murakami Tomoya Kitamura Hiromichi Matsugo Haruhiko Kamiki Ken Oyabu Wataru Sekine Akiko Takenaka-Uema Yuko Sakai-Tagawa Yoshihiro Kawaoka Taisuke Horimoto

PMID36417954 DOI10.3201/eid2812.220801 Year2022 Journal Emerging infectious diseases Emerg Infect Dis Languageeng Publication statusActive

Active Last checked 2026-10-03 03:30:43 Source: NCBI PubMed

Abstract

Surveillance of bat betacoronaviruses is crucial for understanding their spillover potential. We isolated bat sarbecoviruses from Rhinolophus cornutus bats in multiple locations in Japan. These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2, suggesting a narrow host range.

Supporting text Virus Host Location

ACE2 55 angiotensin converting enzyme-2 1 bat 54 betacoronavirus 90 coronaviruses 33 Japan 16 respiratory infections 31 Rhinolophus 3 sarbecovirus 20 SARS-CoV-2 556 SARS-related coronavirus 5 severe acute respiratory syndrome coronavirus 2 20 virus isolation 9 viruses 120 zoonoses 482 Chiroptera 376 Animals 1981 Betacoronavirus 79 Host Specificity 135 Humans 1455 Japan 13 Peptidyl-Dipeptidase A 58

Evidence records

2 total

Zoonotic Surveillance

1 records · 1 evidence types

Evidence type Virus Isolation

1 records

OVE6538 Virus Isolation

Key finding

Bat sarbecoviruses were isolated from Rhinolophus cornutus bats collected in multiple locations in Japan.

Virus

Host

Location

Supporting text

We isolated bat sarbecoviruses from Rhinolophus cornutus bats in multiple locations in Japan.

Functional Mechanism

1 records · 1 evidence types

Evidence type Receptor Usage

1 records

OVE6539 Receptor Usage

Key finding

Bat sarbecoviruses use Rhinolophus cornutus ACE2 but not human ACE2 as the entry receptor.

Virus

Host

Location

Supporting text

These viruses grew efficiently in cells expressing R. cornutus angiotensin converting enzyme-2, but not in cells expressing human angiotensin converting enzyme-2.

Method: cell culture infection assay | receptor expression experiment
Receptors: R. cornutus angiotensin converting enzyme-2 | human angiotensin converting enzyme-2

References

15 references

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