OmniVira

Identification of novel and diverse rotaviruses in rodents and insectivores, and evidence of cross-species transmission into humans.

Kun Li1,2 Xian-Dan Lin3 Kai-Yu Huang4 Bing Zhang5 Mang Shi1,6 Wen-Ping Guo1,2 Miao-Ruo Wang7 Wen Wang1,2 Jian-Guang Xing8 Ming-Hui Li1,2 Wang-Sheng Hong9 Edward C Holmes1,6 Yong-Zhen Zhang1,10
Affiliations 10 institutions
  1. State Key Laboratory of Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, China
  2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China.
  3. Wenzhou Center for Disease Control and Prevention, Wenzhou, Zhejiang Province, China.
  4. The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
  5. State Key Laboratory of Infectious Disease Prevention and Control, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, China.
  6. Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia.
  7. Longquan Center for Disease Control and Prevention, Longquan, Zhejiang Province, China.
  8. Wencheng Center for Disease Control and Prevention, Wencheng, Zhejiang Province, China.
  9. Ruian Center for Disease Control and Prevention, Ruian, Zhejiang Province, China.
  10. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, Zhejiang Province, China. Electronic address: [email protected].
PMID27115729 DOI10.1016/j.virol.2016.04.017 Year2016 Journal Virology Languageeng Statusppublish

Abstract

Rotaviruses are an important cause of severe diarrheal illness in children globally. We characterized rotaviruses sampled in humans, insectivores (shrews) and rodents from urban and rural regions of Zhejiang province, China. Phylogenetic analyses revealed seven genotypic constellations of human rotaviruses with six different combinations of G and P genotypes - G3P[8] (50.06%), G9P[8] (36.16%), G1P[8] (8.92%), G2P[4] (4.63%), G3P[3] (0.12%), and G3P[9] (0.12%). In rodents and shrews sampled from the same locality we identified a novel genotype constellation (G32-P[46]-I24-R18-C17-M17-A28-N17-T19-E24-H19), a novel P genotype (P[45]), and two different AU-1-like rotaviruses associated with a G3P[3] genotype combination. Of particular note was a novel rotavirus from a human patient that was closely related to viruses sampled from rodents in the same region, indicative of a local species jump. In sum, these data are suggestive of the cross-species transmission of rodent rotaviruses into humans and for reassortment among human and animal rotaviruses.

Supporting text Virus Host Location
Evolution 62 Genotype 143 Human infection 8 Reassortment 36 Rodents 12 Rotavirus 66 Shrews 16 Animals 1948 Capsid Proteins 21 Child 42 Child, Preschool 37 China 229 Diarrhea 29 Evolution, Molecular 176 Feces 113 Female 289 Genome, Viral 317 Genotype 137 Humans 1440 Infant 26 Insecta 3 Male 224 Mammals 92 Phylogeny 805

Evidence records

3 total
Zoonotic Surveillance
2 records · 1 evidence types
Evidence type Viral Detection
2 records
OVE2326 Viral Detection
Key finding

Rotaviruses were detected and characterized in humans, shrews, and rodents sampled from urban and rural regions of Zhejiang province, China.

Virus
Rotavirus
Host
human Homo sapiens Natural host shrews Soricidae Natural host rodents Rodentia Natural host
Location
China
Supporting text

We characterized rotaviruses sampled in humans, insectivores (shrews) and rodents from urban and rural regions of Zhejiang province, China.

Method
characterization | genotypic analysis | sampling
Geographic raw
Zhejiang province, China
Country inferred
CHN
OVE2327 Viral Detection
Key finding

Novel rotavirus genotypes including P[45] and a unique genotype constellation G32-P[46]-I24-R18-C17-M17-A28-N17-T19-E24-H19 were detected in rodents and shrews from Zhejiang province, China.

Virus
Rotavirus
Host
rodents Rodentia Natural host shrews Soricidae Natural host
Location
China
Supporting text

In rodents and shrews sampled from the same locality in Zhejiang province, China, we identified a novel genotype constellation (G32-P[46]-I24-R18-C17-M17-A28-N17-T19-E24-H19), a novel P genotype (P[45]), and two different AU-1-like rotaviruses associated with a G3P[3] genotype combination.

Method
genotypic characterization | identification
Geographic raw
Zhejiang province, China
Country inferred
CHN
Genomic Evolution
1 records · 1 evidence types
Evidence type Phylogenetic Evolution
1 records
OVE2328 Phylogenetic Evolution
Key finding

Phylogenetic analysis showed that human rotaviruses from Zhejiang province formed seven distinct genotypic constellations defined by different G and P combinations.

Virus
Rotavirus A
Host
human Homo sapiens Natural host
Location
Not specified
Supporting text

Phylogenetic analyses revealed seven genotypic constellations of human rotaviruses with six different combinations of G and P genotypes.

Genes or proteins
G genotype | P genotype
Analysis methods
phylogenetic analysis